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mmgF [2019-08-07 10:29:23]
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mmgF [2019-08-07 10:29:23]

methylisocitrate lyase
Locus
BSU_24120
Isoelectric point
5.28
Molecular weight
32.94 kDa
Protein length
301 aa Sequence Blast
Gene length
906 bp Sequence Blast
Function
mother cell metabolism
Product
methylisocitrate lyase
Essential
no
E.C.
4.1.3.30
Synonyms
yqiQ,prpB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of branched-chain amino acids
  • Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

    • Gene

    Coordinates
    2,507,298 2,508,203

    The protein

    Catalyzed reaction/ biological activity

  • 2-methyl-isocitrate --> pyruvate + succinate PubMed

    • Protein family

  • isocitrate lyase/PEP mutase superfamily (single member, according to UniProt)

    • Structure

  • 1MUM (enzyme of E. coli), 3KZ2 (the enzyme from B. anthracis)

    • Expression and Regulation

    Operons

    Genes
    mmgA-mmgB-mmgC-mmgD-mmgE-mmgF
    Description
    PubMed

    Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

    • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon

    • Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed
  • strongly expressed during oligotrophic growth PubMed
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    Biological materials

    Mutant

  • MGNA-C374 (yqiQ::erm), available at the NBRP B. subtilis, Japan
  • BKE24120 (mmgF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGACATGAAACAAAATCCCC, downstream forward: _UP4_TAATGCAACAAAAAAAGCCG
  • BKK24120 (mmgF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGACATGAAACAAAATCCCC, downstream forward: _UP4_TAATGCAACAAAAAAAGCCG

    • References

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