mmgF

methylisocitrate lyase

locus: BSU_24120

pI: 5.28

mw: 32.94 kDa

protein length: 301 aa

gene length: 906 bp

function: mother cell metabolism

product: methylisocitrate lyase

essential: no

ec: 4.1.3.30

synonyms: yqiQ,prpB

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.9|Utilization of branched-chain amino acids]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 2,507,298 2,508,203

The protein

Catalyzed reaction/ biological activity

• 2-methyl-isocitrate --> pyruvate + succinate [pubmed|28956599]
• 3-hydroxybutane-1,2,3-tricarboxylate --> pyruvate + succinate (according to UniProt)

Protein family

• isocitrate lyase/PEP mutase superfamily (single member, according to UniProt)

Structure

• [PDB|1MUM] (enzyme of ''E. coli''), [PDB|3KZ2] (the enzyme from ''B. anthracis'')

Expression and Regulation

Operons

• genes: [gene|226100C0AC13BB345DB0F0F309DEB7F91F3770B0|mmgA]-[gene|FB95ABDC8557F26525E8B211595C130A7203ABC0|mmgB]-[gene|F25CDDEBAFC72036664323619F5197D45E86BE9A|mmgC]-[gene|3DB116F7423C31838ED6EE090B94DA5F93A4F40C|mmgD]-[gene|602EAB591A550F68C14F35F6E5F0A91C3B997343|mmgE]-[gene|2F4A025B8E3F14764D24CD7E33F7608C2EB1C42C|mmgF]
  description: [Pubmed|8759838]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190,8759838], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|8759838], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • expressed early during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]) [Pubmed|15699190,8759838]
  • strongly expressed during oligotrophic growth [pubmed|30792386]

Biological materials

Mutant

• MGNA-C374 (yqiQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2372 NBRP B. subtilis, Japan]
• BKE24120 ([gene|2F4A025B8E3F14764D24CD7E33F7608C2EB1C42C|mmgF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGACATGAAACAAAATCCCC, downstream forward: _UP4_TAATGCAACAAAAAAAGCCG
• BKK24120 ([gene|2F4A025B8E3F14764D24CD7E33F7608C2EB1C42C|mmgF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGACATGAAACAAAATCCCC, downstream forward: _UP4_TAATGCAACAAAAAAAGCCG

References

• 12706720,8759838,15699190,28956599