SubtiBank SubtiBank
mmgF
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mmgF

methylisocitrate lyase
Locus
BSU_24120
Isoelectric point
5.28
Molecular weight
32.94 kDa
Protein length
301 aa Sequence Blast
Gene length
906 bp Sequence Blast
Function
mother cell metabolism
Product
methylisocitrate lyase
Essential
no
E.C.
4.1.3.30
Synonyms
yqiQ,prpB

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,507,298 2,508,203

The protein

Catalyzed reaction/ biological activity

  • 2-methyl-isocitrate --> pyruvate + succinate PubMed
  • 3-hydroxybutane-1,2,3-tricarboxylate --> pyruvate + succinate (according to UniProt)
  • Protein family

  • isocitrate lyase/PEP mutase superfamily (single member, according to UniProt)
  • Structure

  • 1MUM (enzyme of E. coli), 3KZ2 (the enzyme from B. anthracis)
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon
  • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed
  • strongly expressed during oligotrophic growth PubMed
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    Biological materials

    Mutant

  • MGNA-C374 (yqiQ::erm), available at the NBRP B. subtilis, Japan
  • BKE24120 (mmgF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGACATGAAACAAAATCCCC, downstream forward: _UP4_TAATGCAACAAAAAAAGCCG
  • BKK24120 (mmgF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGACATGAAACAAAATCCCC, downstream forward: _UP4_TAATGCAACAAAAAAAGCCG
  • References

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