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acoL [2018-07-17 19:22:29]
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acoL [2018-07-17 19:22:29]

acetoin dehydrogenase E3 component (dihydrolipoamide dehydrogenase)
Locus
BSU08090
Isoelectric point
5.27
Molecular weight
48.69 kDa
Protein length
458 aa Sequence Blast
Gene length
1374 bp Sequence Blast
Function
acetoin utilization
Product
acetoin dehydrogenase E3 component (dihydrolipoamide dehydrogenase)
Essential
no
E.C.
1.8.1.4
Synonyms
yfjH

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
882,266 → 883,642

The protein

Catalyzed reaction/ biological activity

  • Protein N(6)-(dihydrolipoyl)lysine + NAD+ = protein N(6)-(lipoyl)lysine + NADH (according to Swiss-Prot)
  • Protein family

  • class-I pyridine nucleotide-disulfide oxidoreductase family (according to Swiss-Prot)
  • Paralogous protein(s)

    Localization

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigL: sigma factor, PubMed, in SigL regulon
  • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • AcoR: activation, (interaction with SigL-containing RNA polymerase) [ PubMed|11274109], in AcoR regulon
  • Fnr: indirect positive regulation, in Fnr regulon
  • Regulation

  • induced by acetoin (AcoR) PubMed
  • expression of the operon is strongly induced during biofilm formation PubMed
  • Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
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    Biological materials

    Mutant

  • MGNA-C351 (acoL::erm), available at the NBRP B. subtilis, Japan
  • BKE08090 (ΔacoL::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCGTTTTCACCTGCTT, downstream forward: _UP4_TAATAAAGGAAAAAGCAGGC
  • BKK08090 (ΔacoL::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCGTTTTCACCTGCTT, downstream forward: _UP4_TAATAAAGGAAAAAGCAGGC
  • Labs working on this gene/protein

  • Michel Debarbouille, Pasteur Institute, Paris, France Homepage
  • References

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