lipA

lipoyl synthase, trigger enzyme

locus: BSU_32330

pI: 8.25

mw: 33.77 kDa

protein length: 298 aa

gene length: 897 bp

function: synthesis of lipoic acid

product: lipoyl synthase,trigger enzyme

essential: no

ec: 2.8.1.8

synonyms: yutB

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.7|Biosynthesis and scavenging of lipoic acid]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.5|Trigger enzymes that control transcription in a yet unknown way]

Gene

Coordinates: 3,320,324 3,321,220

Phenotypes of a mutant

• reduced transformation efficiency [Pubmed|19028902]
• strong inhibition of growth on minimal media [Pubmed|19820084]

The protein

Catalyzed reaction/ biological activity

• [[Fe-S] cluster scaffold protein carrying a second [4Fe-4S]²⁺ cluster] + 4 H⁺ + N⁶-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine --> (R)-N⁶-dihydrolipoyl-L-lysyl-[protein] + 2 5'-deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe³⁺ + 2 hydrogen sulfide + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin] (according to UniProt)
• required for ''[gene|788725411B2E741103603373E43441FD036E1BA0|comEA]'' transcription [Pubmed|19028902]

Protein family

• [SW|Radical SAM superfamily] (according to UniProt)

[SW|Cofactors]

• Fe-S cluster [pubmed|29292548]

Structure

• [PDB|4U0O] (from ''Thermosynechococcus elongatus'', 48% identity) [Pubmed|25100160]

Expression and Regulation

Operons

• genes: [gene|2EC820F54911603A99444DC922E41C60AA526EB2|lipA]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-B578 (yutB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1577 NBRP B. subtilis, Japan]
• BKE32330 ([gene|2EC820F54911603A99444DC922E41C60AA526EB2|lipA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTCCCATCATCTCCAAT, downstream forward: _UP4_TAATGCCAAAACGCCAGATC
• BKK32330 ([gene|2EC820F54911603A99444DC922E41C60AA526EB2|lipA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTCCCATCATCTCCAAT, downstream forward: _UP4_TAATGCCAAAACGCCAGATC

References

Reviews

• 27074917

Original publications

• 18957862,19028902,19820084,25100160