lgt

prolipoprotein diacylglyceryl transferase

Locus: BSU_34990

Isoelectric point: 8.96

Molecular weight: 30.47 kDa

Protein length: 269 aa

Gene length: 810 bp

Function: lipomodification of lipoproteins

Product: prolipoprotein diacylglyceryl transferase

Essential: no

E.C.: 2.4.99.-

Synonyms: gerF,yvoC

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Protein modification → Protein maturation
• Lifestyles → Sporulation → Germination → Additional germination proteins
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,593,419 3,594,228

Phenotypes of a mutant

• reduced ability of spores to germinate with alanine due ti reduced activity of the GerAA-GerAB-GerAC germinant receptor PubMed
• inactivation of lgt reduces sporulation efficiency to 7.7% that of wild type cells PubMed

The protein

Catalyzed reaction/ biological activity

• 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + L-cysteinyl-[prolipoprotein] --> H⁺ + S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[prolipoprotein] + sn-glycerol 1-phosphate (according to UniProt)

Protein family

• lgt family (single member, according to UniProt)

Structure

• 5AZB (from E. coli, 29% identity) PubMed

Localization

• membrane associated PubMed

Expression and Regulation

Operons

• Genes: hprK-lgt-yvoD-yvoE-yvoF
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

Biological materials

Mutant

• MGNA-A388 (lgt::erm), available at the NBRP B. subtilis, Japan
• GP851 (Δlgt-yvoD-yvoE-yvoF)::aphA3), available in Jörg Stülke's lab
• 1G22 ( lgt::erm), PubMed, available at BGSC
• BKE34990 (Δlgt::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGCTTCATTCATTTAACGCC, downstream forward: _UP4_TAGACGTCAACAAAGGGGGA
• BKK34990 (Δlgt::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGCTTCATTCATTTAACGCC, downstream forward: _UP4_TAGACGTCAACAAAGGGGGA

References

Reviews

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Original publications

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Lgt in other organisms

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