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gutB [2018-05-23 18:37:31]
function
glucitol utilization
product
D-sorbitol dehydrogenase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.11|Utilization of glucitol]Gene
Coordinates
667,466 → 668,527
The protein
Catalyzed reaction/ biological activity
L-iditol + NAD+ = L-sorbose + NADH (according to Swiss-Prot)Protein family
zinc-containing alcohol dehydrogenase family (according to Swiss-Prot)Structure
[PDB|1PL7] (the human enzyme, 42% identity)Expression and Regulation
Operons
genes
[gene|2E9D10B0FA5685800007222A5AAD71E975675DB1|gutB]-[gene|B13F7C35FBD55C674E5B19836D32279E7C1A5520|gutP]-[gene|0C4F0C9EF18FB4E9BB5BC3F53F7DDF5F32B51FA5|fruC]
description
[Pubmed|22383849]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8195086], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|926BCA197F259558F72FDCC73998497B9B167D22|GutR]: activation, [Pubmed|8195086], in [regulon|926BCA197F259558F72FDCC73998497B9B167D22|GutR regulon]regulation
induced by glucitol ([protein|926BCA197F259558F72FDCC73998497B9B167D22|GutR]) [Pubmed|8195086]view in new tabBiological materials
Mutant
BKE06150 (Δ[gene|2E9D10B0FA5685800007222A5AAD71E975675DB1|gutB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGGCAAGTTCCTTTCT, downstream forward: _UP4_TGAGTGAACAGGGAGGATCTBKK06150 (Δ[gene|2E9D10B0FA5685800007222A5AAD71E975675DB1|gutB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGGCAAGTTCCTTTCT, downstream forward: _UP4_TGAGTGAACAGGGAGGATCTReferences