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pucL [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]
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pucL [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

uricase, urate oxidase
locus
BSU32450
pI
5.48
mw
56.00 kDa
function
purine utilization
product
uricase
essential
no
ec
1.7.3.3
synonyms
yunL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.1|Utilization of nucleotides]

    • [SW|Categories] containing this gene/protein

  • [SW|utilization of nucleotides]

    • This gene is a member of the following [SW|regulons]

  • [SW|PucR regulon], [SW|TnrA regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    3,333,162 -> 3,334,646

    The protein

    Catalyzed reaction/ biological activity

  • 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate = (S)-allantoin + CO2 (according to Swiss-Prot)

    • Structure

  • [PDB|1J2G] (with 8-Azaxanthine from ''Bacillus sp.'', 66% identity, 79% similarity)

    • Expression and Regulation

    Operon

  • ''[protein|search|pucR]-[protein|search|pucJ]-[protein|search|pucK]-[protein|search|pucL]-[protein|search|pucM]'' [Pubmed|12823818]
  • ''[protein|search|pucJ]-[protein|search|pucK]-[protein|search|pucL]-[protein|search|pucM]'' [Pubmed|12029039]

    • [SW|Sigma factor]

  • ''[protein|search|pucJ]'': [protein|search|SigA] [Pubmed|12029039]

    • Regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]
  • induced in the presence of purine nucleotides (inducer: allantoin) ([protein|search|PucR]) [Pubmed|12029039]

    • Regulatory mechanism

  • [protein|search|TnrA]: transcription activation [Pubmed|12823818]
  • [protein|search|PucR]: transcription activation [Pubmed|12029039]

    • References

  • 11344136,25755103,12823818,12029039,20168977