ndoA [2017-8-11 16:0:3]

RNase EndoA, MazF family toxin, UACAU-specific mRNA interferase

locus: BSU04660

pI: 4.89

mw: 12.84 kDa

protein length: 116 aa

gene length: 348 bp

function: unknown

product: mRNA interferase

essential: no

synonyms: ydcE, mazF

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.2|Endoribonucleases]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.17|Toxins, antitoxins and immunity against toxins]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.17|Toxins, antitoxins and immunity against toxins] → [category|SW 4.3.17.2|Type 2 TA systems]

Gene

Coordinates: 518,943 → 519,293

The protein

Catalyzed reaction/ biological activity

• specifically cleaves a five-base sequence, UACAU [Pubmed|21763692]

Protein family

• pemK family (according to Swiss-Prot)

Effectors of protein activity

• the activity is inhibited by the interaction with [protein|052BD59112DBE2F1DAC9DA5A7B0EA8DAF8D6FC47|NdoAI] [Pubmed|17416361]

Structure

• [PDB|1NE8] [Pubmed|14517982]
• [PDB|4MDX] (NdoA-RNA) [Pubmed|24120662]
• [PDB|4ME7] ([protein|2E5A557E417CB355331CC0555D46A79793EB90B3|NdoA]-[protein|052BD59112DBE2F1DAC9DA5A7B0EA8DAF8D6FC47|NdoAI] complex) [Pubmed|24120662]

Expression and Regulation

Operons

• genes: [gene|052BD59112DBE2F1DAC9DA5A7B0EA8DAF8D6FC47|ndoAI]-[gene|2E5A557E417CB355331CC0555D46A79793EB90B3|ndoA]
  description: [Pubmed|15882409]

  additional information

  • An [ncRNA|search|antisense RNA] is predicted for '[protein|search|ndoA]' [PubMed|20525796]

• genes: [gene|CB0B6C69CB5CFF6FE30C3F95991F309C8DE1E344|alr]-[gene|052BD59112DBE2F1DAC9DA5A7B0EA8DAF8D6FC47|ndoAI]-[gene|2E5A557E417CB355331CC0555D46A79793EB90B3|ndoA]
  description: [pubmed|22383849]

References

• 15882409,17416361,20525796,21763692,21897862,23378533,14517982,24120662,24489751,23736285,28017721