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ndoA
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ndoA

RNase EndoA, MazF family toxin, UACAU-specific mRNA interferase
locus
BSU_04660
pI
4.89
mw
12.84 kDa
protein length
116 aa Sequence Blast
gene length
351 bp Sequence Blast
function
unknown
product
mRNA interferase
essential
no
synonyms
ydcE,mazF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.2|Endoribonucleases]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.17|Toxins, antitoxins and immunity against toxins]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.17|Toxins, antitoxins and immunity against toxins] → [category|SW 4.3.17.2|Type 2 TA systems]

    • Gene

    Coordinates
    518,943 519,293

    The protein

    Catalyzed reaction/ biological activity

  • specifically cleaves a five-base sequence, UACAU [Pubmed|21763692]

    • Protein family

  • PemK/MazF family (single member, according to UniProt)

    • Effectors of protein activity

  • the activity is inhibited by the interaction with [protein|052BD59112DBE2F1DAC9DA5A7B0EA8DAF8D6FC47|NdoAI] [Pubmed|17416361]

    • Structure

  • [PDB|1NE8] [Pubmed|14517982]
  • [PDB|4MDX] (NdoA-RNA) [Pubmed|24120662]
  • [PDB|4ME7] ([protein|2E5A557E417CB355331CC0555D46A79793EB90B3|NdoA]-[protein|052BD59112DBE2F1DAC9DA5A7B0EA8DAF8D6FC47|NdoAI] complex) [Pubmed|24120662]

    • Expression and Regulation

    Operons

    genes
    [gene|052BD59112DBE2F1DAC9DA5A7B0EA8DAF8D6FC47|ndoAI]-[gene|2E5A557E417CB355331CC0555D46A79793EB90B3|ndoA]
    description
    [Pubmed|15882409]

    additional information

  • An [ncRNA|search|antisense RNA] is predicted for '[protein|search|ndoA]' [PubMed|20525796]
    • view in new tab

    genes
    [gene|CB0B6C69CB5CFF6FE30C3F95991F309C8DE1E344|alr]-[gene|052BD59112DBE2F1DAC9DA5A7B0EA8DAF8D6FC47|ndoAI]-[gene|2E5A557E417CB355331CC0555D46A79793EB90B3|ndoA]
    description
    [pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • BKE04660 ([gene|2E5A557E417CB355331CC0555D46A79793EB90B3|ndoA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCGCCGCGTTTCACAATCA, downstream forward: _UP4_TAGACATATTTGCAGGTTGC
  • BKK04660 ([gene|2E5A557E417CB355331CC0555D46A79793EB90B3|ndoA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCGCCGCGTTTCACAATCA, downstream forward: _UP4_TAGACATATTTGCAGGTTGC

    • References

  • 15882409,17416361,20525796,21763692,21897862,23378533,14517982,24120662,24489751,23736285,28017721