nasA

nitrate transporter

Locus: BSU_03330

Isoelectric point: 10.49

Molecular weight: 45.91 kDa

Protein length: 401 aa

Gene length: 1206 bp

Function: nitrate uptake

Product: nitrate transporter

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Uptake of other small ions
• Metabolism → Amino acid/ nitrogen metabolism → Utilization of nitrogen sources other than amino acids → Utilization of nitrate/ nitrite
• Groups of genes → Membrane proteins

Gene

Coordinates: 362,937 364,142

The protein

Protein family

• major facilitator superfamily (according to UniProt)
• Nitrate/nitrite porter (TC 2.A.1.8) family (with NarK, according to UniProt)

Paralogous protein(s)

• NarK

Structure

• 4U4T (NarK from E. coli, 24% identity) PubMed

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: nasA
  Description: PubMed

  Regulatory mechanism

  • TnrA: activation, PubMed, in TnrA regulon

  Regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

Biological materials

Mutant

• 1A972 ( nasA::phleo), PubMed, available at BGSC
• BKE03330 (nasA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAGATTCCCCTTTCTAG, downstream forward: _UP4_TAGGATTTTGACGGACACGC
• BKK03330 (nasA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAGATTCCCCTTTCTAG, downstream forward: _UP4_TAGGATTTTGACGGACACGC

References

Reviews

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Original publications

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