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ald
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ald

L-alanine dehydrogenase
locus
BSU_31930
pI
5.12
mw
39.53 kDa
protein length
378 aa Sequence Blast
gene length
1137 bp Sequence Blast
function
alanine utilization
product
L-alanine dehydrogenase
essential
no
ec
1.4.1.1
synonyms
spoVN
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.7|Utilization of alanine/ serine]

    • Gene

    Coordinates
    3,278,325 3,279,461

    Phenotypes of a mutant

  • [SW|sporulation] defect [Pubmed|22797752]

    • The protein

    Catalyzed reaction/ biological activity

  • H2O + L-alanine + NAD+ --> H+ + NADH + NH4+ + pyruvate (according to UniProt)

    • Protein family

  • AlaDH/PNT family (single member, according to UniProt)

    • [SW|Cofactors]

  • NADH [Pubmed|26202482]

    • Structure

  • [PDB|2EEZ] (from ''Thermus thermophilus hb8 mutant'', 55% identity, 66% similarity)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|2D346CF412BA32EE18111DA930CFA1306F95024B|ald]-[gene|F001D5A254F0E175B4B3A0B54FC4B40AA230997E|yukJ]

    description

  • [pubmed|22383849]
    • view in new tab

    genes
    [gene|2D346CF412BA32EE18111DA930CFA1306F95024B|ald]
    description
    [Pubmed|8226620]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8226620], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|A987E1510578E8DE822C2019488D457CB7CFBE73|AdeR]: activation, [Pubmed|22797752], in [regulon|A987E1510578E8DE822C2019488D457CB7CFBE73|AdeR regulon]

    • regulation

  • induced by alanine ([protein|search|AdeR]) [Pubmed|22797752,8226620]
    • view in new tab

    Biological materials

    Mutant

  • BKE31930 ([gene|2D346CF412BA32EE18111DA930CFA1306F95024B|ald]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTGTCTCCTCCTGTA, downstream forward: _UP4_TAATTCACAATAAGCTTGCA
  • BKK31930 ([gene|2D346CF412BA32EE18111DA930CFA1306F95024B|ald]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTGTCTCCTCCTGTA, downstream forward: _UP4_TAATTCACAATAAGCTTGCA

    • References

  • 19114526,9168598,17183219,8226620,22797752,26202482,29302032