ylaG [2018-10-12 17:39:35]

similar to GTP-binding elongation factor

locus: BSU14770

pI: 4.97

mw: 68.21 kDa

protein length: 612 aa

gene length: 1836 bp

function: unknown

product: unknown

essential: no

synonyms: bipA

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.11|Translation factors]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.5|Cold stress proteins]

Gene

Coordinates: 1,546,121 → 1,547,959

The protein

Catalyzed reaction/ biological activity

• in E. coli, the corresponding protein (BipA) ribosomal protein [protein|20485A785D3AF82889F2018D3EA5B5FA33E64E95|L6] into the nascent large ribosomal subunit [pubmed|30305394]

Protein family

• TypA subfamily (according to Swiss-Prot)

Structure

• [PDB|4ZCM]

Expression and Regulation

Operons

• genes: [gene|2D0A95A37214E9BF58E2DE732DE9F32AAF9C1CD3|ylaG]-[gene|8DE3D48909BDBD2091432047E79063E9EEE63115|ylaH]
  description: [pubmed|22383849]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

Biological materials

Mutant

• MGNA-A536 (ylaG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/536 NBRP B. subtilis, Japan]
• BKE14770 (Δ[gene|2D0A95A37214E9BF58E2DE732DE9F32AAF9C1CD3|ylaG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTTCATCTCCTAAAA, downstream forward: _UP4_TAATCTCGTGCAGAATTTGA
• BKK14770 (Δ[gene|2D0A95A37214E9BF58E2DE732DE9F32AAF9C1CD3|ylaG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTTCATCTCCTAAAA, downstream forward: _UP4_TAATCTCGTGCAGAATTTGA

References

• 11948165,12399512,30305394