spo0A [2017-07-24 12:51:07]

phosphorelay regulator, initiation of sporulation, coordinates DNA replication and initiation of sporulation by binding to sites close to the oriC

Locus: BSU24220

Isoelectric point: 5.99

Molecular weight: 29.54 kDa

Protein length: 267 aa

Gene length: 801 bp

Function: initiation of sporulation

Product: phosphorelay response regulator

Essential: no

Synonyms: spo0C, spo0G, spoIIL, sof-1

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → Transcription factors and their control → Two-component system response regulators
• Information processing → Regulation of gene expression → phosphorelay
• Information processing → Regulation of gene expression → phosphorelay → The ultimate target
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Regulation
• Lifestyles → Sporulation → phosphorelay
• Lifestyles → Sporulation → phosphorelay → The ultimate target
• Groups of genes → Phosphoproteins → Phosphorylation on an Asp residue

Gene

Coordinates: 2,518,023 → 2,518,826

Phenotypes of a mutant

• inactivation of spo0A restores beta-lactam resistance in a sigM mutant PubMed
• altered cell death pattern in colonies PubMed

The protein

Catalyzed reaction/ biological activity

• activation of gene expression at the onset of sporulation (Spo0A regulon)
• coordination between DNA replication and initiation of sporulation by binding to sites close to the oriC (this limits re-initiation of replication) PubMed
• required for biofilm formation PubMed
• required for sliding PubMed

Modification

• receives phosphorylation from Spo0B, dephosphorylation by Spo0E, direct phosphorylation by KinC upon potassium leakage PubMed

Effectors of protein activity

• the phosphorylation state affects DNA binding activity
• interaction with MecA inhibits the transcriptional activity of Spo0A∼P PubMed

Structure

• 1QMP (with phosphorylated aspartate, Geobacillus stearothermophilus), 1FC3 (trans-activation domain, Geobacillus stearothermophilus), 1LQ1 (complex with DNA)

Expression and Regulation

Operons

• Genes: spo0A
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigH: sigma factor, PubMed, in SigH regulon

  Regulatory mechanism

  • Spo0A: repression, PubMed, in Spo0A regulon

  Regulation

  • expression increases when drowth rate declines PubMed

Biological materials

Mutant

• MGNA-C448 (spo0A::erm), available at the NBRP B. subtilis, Japan
• BKG9 (spo0A::aphA3), available in Jörg Stülke's lab
• SS995 (spo0A::ery) PubMed

Labs working on this gene/protein

• Tony Wilkinson, York University, U.K. homepage
• Imrich Barak, Slovak Academy of Science, Bratislava, Slovakia homepage
• Charles Moran, Emory University, NC, USA homepage

References

Reviews

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The Spo0A regulon

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Other original Publications

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