yxkC

unknown

Locus: BSU_38850

Isoelectric point: 9.29

Molecular weight: 22.00 kDa

Protein length: 213 aa

Gene length: 642 bp

Function: unknown

Product: unknown

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Groups of genes → Secreted proteins
• Groups of genes → Proteins of unknown function

Gene

Coordinates: 3,989,232 3,989,873

The protein

Domains

• DUF4352 (aa 46 ... 175)(according to UniProt)

Structure

• 4LES (from B. anthracis, corresponds to aa 79 ... 213, 30% identity)

Localization

• extracellular (signal peptide) PubMed
• membrane PubMed

Expression and Regulation

Operons

• Genes: yxkC
  Description: PubMed

  Sigma factors

  • SigD: sigma factor, PubMed, in SigD regulon

  Regulatory mechanism

  • TnrA: activation, PubMed, in TnrA regulon

  Regulation

  • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

Biological materials

Mutant

• MGNA-B737 (yxkC::erm), available at the NBRP B. subtilis, Japan
• BKE38850 (yxkC::erm, available in the BGSC and in Jörg Stülke's lab) PubMed
• GP1824 (yxkC::erm, available in Jörg Stülke's lab)
• BKE38850 (yxkC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAAAAAGTTACGTTGAT, downstream forward: _UP4_TAACAGCTAACAAGGGTGCC
• BKK38850 (yxkC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAAAAAGTTACGTTGAT, downstream forward: _UP4_TAACAGCTAACAAGGGTGCC

References

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