rplN [2019-07-08 10:39:53]

ribosomal protein

Locus: BSU01260

Isoelectric point: 10.40

Molecular weight: 13.01 kDa

Protein length: 122 aa

Gene length: 369 bp

Function: translation

Product: ribosomal protein L14

Essential: yes

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → Ribosomal proteins
• Groups of genes → Essential genes
• Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue
• Groups of genes → Universally conserved proteins

Gene

Coordinates: 140,451 140,819

Phenotypes of a mutant

• essential PubMed

The protein

Protein family

• universal ribosomal protein uL14 family (single member, according to UniProt)

Modification

• phosphorylated on Arg-7 PubMed

Structure

• 1WHI (Geobacillus stearothermophilus)
• 3J9W (the ribosome) PubMed

Expression and Regulation

Operons

• Genes: rpsJ-rplC-rplD-rplW-rplB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ-rplN-rplX-rplE-rpsN-rpsH-rplF-rplR-rpsE-rpmD-rplO-secY-adk-map-ybzG-infA-rpmJ-rpsM-rpsK-S56-rpoA-rplQ
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed (two promoters, about 140bp and 200bp upstream of the start codon), in SigA regulon

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

  Regulation

  • strongly repressed in response to glucose starvation in M9 medium PubMed
  • likely autorepression of operon expression upon binding of excess of one ribosomal protein to the untranslated region of the mRNA PubMed

  Additional information

  • term-seq has identified a potential novel regulatory RNA element (protein-dependent leader) including an intrinsic transcription terminator upstream of rpsJ PubMed

Additional information

• the mRNA is cleaved by RNase III PubMed

References

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