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abfA
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abfA

alpha-L-arabinofuranosidase
Locus
BSU_28720
Isoelectric point
5.34
Molecular weight
56.89 kDa
Protein length
500 aa Sequence Blast
Gene length
1503 bp Sequence Blast
Function
arabinan degradation
Product
alpha-L-arabinofuranosidase
Essential
no
E.C.
3.2.1.55
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,938,330 2,939,832

The protein

Catalyzed reaction/ biological activity

  • Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides (according to UniProt)
  • Protein family

  • glycosyl hydrolase 51 family (with Abf2, according to UniProt)
  • Structure

  • 1QW8 (complex with Ara-alpha-Xyl, Geobacillus stearothermophilus), 1PZ3 (Geobacillus stearothermophilus)
  • Localization

  • forms hexamers PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • AraR: repression, PubMed, in AraR regulon
  • Regulation

  • induced by arabinose (AraR) PubMed
  • Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed
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    Biological materials

    Mutant

  • BKE28720 (abfA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTCATCACACGTTTCCTCC, downstream forward: _UP4_TAAGAATAGCAAAGCCGGAG
  • BKK28720 (abfA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTCATCACACGTTTCCTCC, downstream forward: _UP4_TAAGAATAGCAAAGCCGGAG
  • References

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