abfA

alpha-L-arabinofuranosidase

Locus: BSU_28720

Isoelectric point: 5.34

Molecular weight: 56.89 kDa

Protein length: 500 aa

Gene length: 1503 bp

Function: arabinan degradation

Product: alpha-L-arabinofuranosidase

Essential: no

E.C.: 3.2.1.55

Synonyms

Genomic Context

Loading

Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of arabinan/ arabinose/ arabitol

Gene

Coordinates: 2,938,330 2,939,832

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides (according to UniProt)

Protein family

• glycosyl hydrolase 51 family (with Abf2, according to UniProt)

Structure

• 1QW8 (complex with Ara-alpha-Xyl, Geobacillus stearothermophilus), 1PZ3 (Geobacillus stearothermophilus)

Localization

• forms hexamers PubMed

Expression and Regulation

Operons

• Genes: araA-araB-araD-araL-araM-araN-araP-araQ-abfA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • AraR: repression, PubMed, in AraR regulon

  Regulation

  • induced by arabinose (AraR) PubMed

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

Mutant

• BKE28720 (abfA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTCATCACACGTTTCCTCC, downstream forward: _UP4_TAAGAATAGCAAAGCCGGAG
• BKK28720 (abfA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTCATCACACGTTTCCTCC, downstream forward: _UP4_TAAGAATAGCAAAGCCGGAG

References

Loading