SubtiBank SubtiBank
yloU
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yloU

similar to alkaline-shock protein
Locus
BSU_15830
Isoelectric point
7.08
Molecular weight
13.05 kDa
Protein length
120 aa Sequence Blast
Gene length
363 bp Sequence Blast
Function
unknown
Product
unknown
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Groups of genes → Proteins of unknown function

    • Gene

    Coordinates
    1,656,064 1,656,426

    The protein

    Protein family

  • asp23 family (with YqhY, according to UniProt)

    • Paralogous protein(s)

  • YqhY

    • Expression and Regulation

    Operons

    Genes
    yloU-fakA
    Description
    PubMed
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    Biological materials

    Mutant

  • MGNA-B375 (yloU::erm), available at the NBRP B. subtilis, Japan
  • GP1465 (yloU-cat ), available in Jörg Stülke's lab PubMed
  • GP1467 (yloU-fakA-cat ), available in Jörg Stülke's lab PubMed
  • BKE15830 (yloU::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACACTAGTTCCTCCTTCGA, downstream forward: _UP4_AACCCGTAGTTAGGAGGAGT
  • BKK15830 (yloU::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACACTAGTTCCTCCTTCGA, downstream forward: _UP4_AACCCGTAGTTAGGAGGAGT

    • Expression vectors

  • GP1476 (chromosomal yloU-Strep fusion, aphA3), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab
  • pGP1330 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
  • pGP1324 (N-terminal His-tag, in pWH844), available in Jörg Stülke's lab

    • GFP fusion

  • GP1472 (spc, based on pBP43), available in Jörg Stülke's lab PubMed

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • References

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