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racX [2017-09-16 18:13:01]
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racX [2017-09-16 18:13:01]

amino acid racemase, production of non-canonical D-amino acids
locus
BSU34430
pI
5.40
mw
25.14 kDa
protein length
227 aa Sequence Blast
gene length
681 bp Sequence Blast
function
production of non-canonical D-amino acids
product
amino acid racemase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]

    • Gene

    Coordinates
    3,533,419 → 3,534,102

    The protein

    Protein family

  • aspartate/glutamate racemases family (according to Swiss-Prot)

    • Structure

  • [PDB|1JFL] (30% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|FE672C27CF019E18A526DE8E6779788DDE54056E|pbpE]-[gene|2ABCC493C6CA847A7BC65B1200A9B6490621462A|racX]
    description
    [Pubmed|8491712]

    sigma factors

  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|8491712,12207695], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]

    • regulation

  • induced by cell wall stress ([protein|search|SigW]) [Pubmed|8491712,12207695]
    • view in new tab

    Biological materials

    Mutant

  • BKE34430 (Δ[gene|2ABCC493C6CA847A7BC65B1200A9B6490621462A|racX]::erm trpC2), available at [http://www.bgsc.org/getdetail.php?bgscid=BSU34430 BGSC], upstream reverse: 
    _UP1_TCCGATCATCAAATTCATTC
    , downstream forward: 
    _UP4_TAGCTTCAGACAAGGACTGT
  • BKK34430 (Δ[gene|2ABCC493C6CA847A7BC65B1200A9B6490621462A|racX]::kan trpC2), available at [http://www.bgsc.org/getdetail.php?bgscid=BSU34430 BGSC], upstream reverse: 
    _UP1_TCCGATCATCAAATTCATTC
    , downstream forward: 
    _UP4_TAGCTTCAGACAAGGACTGT

    • References

  • 8491712,19063962,9987136,7592498,20817675,28894939