racX

amino acid racemase, production of non-canonical D-amino acids

locus: BSU_34430

pI: 5.40

mw: 25.14 kDa

protein length: 227 aa

gene length: 684 bp

function: production of non-canonical D-amino acids

product: amino acid racemase

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]

Gene

Coordinates: 3,533,419 3,534,102

The protein

Catalyzed reaction/ biological activity

• L-α-amino acid --> D-α-amino acid (according to UniProt)
• LL-2,6-diaminoheptanedioate --> meso-2,6-diaminopimelate(according to UniProt)
• L-lysine --> D-lysine (according to UniProt)
• L-arginine --> D-arginine (according to UniProt)
• L-ornithine --> D-ornithine (according to UniProt)
• L-histidine --> D-histidine (according to UniProt)
• L-alanine --> D-alanine (according to UniProt)
• L-tyrosine --> D-tyrosine (according to UniProt)
• L-phenylalanine --> D-phenylalanine (according to UniProt)
• L-serine --> D-serine (according to UniProt)
• L-glutamine --> D-glutamine (according to UniProt)
• L-methionine --> D-methionine (according to UniProt)
• L-asparagine --> D-asparagine (according to UniProt)
• L-homoserine --> D-homoserine (according to UniProt)

Protein family

• aspartate/glutamate racemases family (with [protein|36FF9F097682D8BA8D7359BFEAF71ACE8E9E90D8|RacE] and [protein|BBEE8CEC868D3B781BEA50C45935BC810EA7A4FB|YrpC], according to UniProt)

Structure

• [PDB|1JFL] (from Pyrococcus horikoshii, 30% identity) [pubmed|12051922]

Expression and Regulation

Operons

• genes: [gene|FE672C27CF019E18A526DE8E6779788DDE54056E|pbpE]-[gene|2ABCC493C6CA847A7BC65B1200A9B6490621462A|racX]
  description: [Pubmed|8491712]

  sigma factors

  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|8491712,12207695], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]

  regulation

  • induced by cell wall stress ([protein|search|SigW]) [Pubmed|8491712,12207695]

Biological materials

Mutant

• BKE34430 ([gene|2ABCC493C6CA847A7BC65B1200A9B6490621462A|racX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCCGATCATCAAATTCATTC, downstream forward: _UP4_TAGCTTCAGACAAGGACTGT
• BKK34430 ([gene|2ABCC493C6CA847A7BC65B1200A9B6490621462A|racX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCCGATCATCAAATTCATTC, downstream forward: _UP4_TAGCTTCAGACAAGGACTGT

References

• 8491712,19063962,9987136,7592498,20817675,28894939,12051922