nadF

NAD kinase

locus: BSU_11610

pI: 5.99

mw: 29.86 kDa

protein length: 266 aa

gene length: 801 bp

function: generation of NADP from NAD

product: inorganic polyphosphate/ATP-NAD kinase

essential: yes

ec: 2.7.1.23

synonyms: yjbN,ppnK,ppnKA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.11|Biosynthesis of NAD(P)]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 1,237,660 1,238,460

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP + NAD⁺ --> ADP + H⁺ + NADP⁺ (according to UniProt)

Protein family

• NAD kinase family (with [protein|B729EFC0BF0D75765C2D2B0B4F397E0AEE11F1E7|YtdI], according to UniProt)

Paralogous protein(s)

• [protein|B729EFC0BF0D75765C2D2B0B4F397E0AEE11F1E7|YtdI]

Effectors of protein activity

• allosterically activated by quinolinic acid

Structure

• [PDB|2I1W] (from ''Listeria monocytogenes'', 60% identity) [Pubmed|17686780]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|E5A57660B69F9E9BC52FD9C8A5E3C8786CE96728|yjbL]-[gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB]-[gene|2A2640A0501ECEA44A3C0C4842FAC053FB422A1F|nadF]-[gene|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO]
  description: [Pubmed|18067544]

  regulation

  • expressed during exponential growth

Biological materials

Mutant

• MGNA-B160 (yjbN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1159 NBRP B. subtilis, Japan]
• 1A825 ( ''nadF''::''erm''), [Pubmed|12682299], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A825&Search=1A825 BGSC]

References

• 12897004,18067544,17686780