ybgE [2018-09-07 08:26:49]

branched-chain amino acid aminotransferase

Locus: BSU02390

Isoelectric point: 4.95

Molecular weight: 39.52 kDa

Protein length: 356 aa

Gene length: 1068 bp

Function: biosynthesis of branched-chain amino acids

Product: branched-chain amino acid aminotransferase

Essential: no

E.C.: 2.6.1.42

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of branched-chain amino acids

Gene

Coordinates: 259,016 → 260,086

The protein

Catalyzed reaction/ biological activity

• l-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate (according to Swiss-Prot)

Protein family

• class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

Paralogous protein(s)

• YwaA

Structure

• 3HT5 (from Mycobacterium tuberculosis, 48% identity) PubMed

Expression and Regulation

Operons

• Genes: ybgE
  Description: PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, in CodY regulon

  Regulation

  • repressed by casamino acids PubMed

Biological materials

Mutant

• MGNA-B939 (ybgE::erm), available at the NBRP B. subtilis, Japan
• a ybgE mutant and a bcd ybgE ywaA triple mutant are available in Linc Sonenshein's lab
• BKE02390 (ΔybgE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATCTAATCAGCACACCTT, downstream forward: _UP4_TGAAAATCGAAAAAGAACCT
• BKK02390 (ΔybgE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATCTAATCAGCACACCTT, downstream forward: _UP4_TGAAAATCGAAAAAGAACCT

LacZ fusion

• pGP248 (in pAC5), available in Jörg Stülke's lab

References

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