branched-chain amino acid aminotransferase
function
biosynthesis of branched-chain amino acids
product
branched-chain amino acid aminotransferase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.12|Biosynthesis/ acquisition of branched-chain amino acids][SW|Categories] containing this gene/protein
[SW|biosynthesis/ acquisition of amino acids]This gene is a member of the following [SW|regulons]
[SW|CodY regulon]Gene
Coordinates on the chromosome (coding sequence)
259,016 -> 260,086
The protein
Catalyzed reaction/ biological activity
l-leucine 2-oxoglutarate = 4-methyl-2-oxopentanoate L-glutamate (according to Swiss-Prot) Protein family
class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)Paralogous protein(s)
[protein|search|YwaA]Structure
[PDB|3HT5] (from ''Mycobacterium tuberculosis'', 48% identity) [Pubmed|19923721]Expression and Regulation
Operon
''ybgE'' [Pubmed|15060025]Regulation
repressed by casamino acids [Pubmed|12107147]repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455]Regulatory mechanism
[protein|search|CodY]: transcription repression [Pubmed|12618455,21699902,18083814]Biological materials
Mutant
a ''ybgE'' mutant and a ''[protein|search|bcd] [protein|search|ybgE] [protein|search|ywaA]'' triple mutant are available in [SW|Linc Sonenshein]'s lablacZ fusion
pGP248 (in [protein|search|pAC5]), available in [SW|Jörg Stülke]'s labReferences
18083814,12618455,12670965,15060025,12107147,21699902,24163341,19923721 
