SubtiBank SubtiBank
purB [2018-06-29 17:48:44]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

purB [2018-06-29 17:48:44]

adenylsuccinate lyase
Locus
BSU06440
Isoelectric point
5.82
Molecular weight
49.32 kDa
Protein length
431 aa Sequence Blast
Gene length
1293 bp Sequence Blast
Function
purine biosynthesis
Product
adenylsuccinate lyase
Essential
no
E.C.
4.3.2.2
Synonyms

Genomic Context

      
Loading

Categories containing this gene/protein

Gene

Coordinates
700,232 → 701,527

Phenotypes of a mutant

  • poorly transformable PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • N(6)-(1,2-dicarboxyethyl)AMP = fumarate AMP (according to Swiss-Prot)
  • Protein family

  • Adenylosuccinate lyase subfamily (according to Swiss-Prot)
  • Structure

    Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • belongs to the 100 most abundant proteins PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • G-box: termination, (riboswitch) PubMed, in G-box
  • PurR: repression, (molecular inducer: PRPP) PubMed, in PurR regulon
  • Regulation

  • expression activated by glucose (4.4 fold) PubMed
  • view in new tab

    Biological materials

    Mutant

  • BKE06440 (ΔpurB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTCAGGTCTTGAATAACGTT, downstream forward: _UP4_TAGAAGAAGCTTTTAGCGGC
  • BKK06440 (ΔpurB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTCAGGTCTTGAATAACGTT, downstream forward: _UP4_TAGAAGAAGCTTTTAGCGGC
  • References

    Loading