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yitU
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yitU

5'-nucleotidase, dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate (minor activity)
Locus
BSU_11140
Isoelectric point
4.67
Molecular weight
30.48 kDa
Protein length
270 aa Sequence Blast
Gene length
813 bp Sequence Blast
Function
dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate (minor activity)
Product
5'-nucleotidase
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,190,490 1,191,302

The protein

Catalyzed reaction/ biological activity

  • dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate (minor activity) PubMed
  • 5-amino-6-(5-phospho-D-ribitylamino)uracil + H2O --> 5-amino-6-(D-ribitylamino)uracil + phosphate (according to UniProt)
  • Protein family

  • HAD superfamily (according to UniProt) PubMed
  • Cof family (according to UniProt)
  • Structure

  • 1RKQ (YidA from E. coli, 31% identity)
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigG: sigma factor, PubMed, in SigG regulon
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    Biological materials

    Mutant

  • MGNA-B185 (yitU::erm), available at the NBRP B. subtilis, Japan
  • BKE11140 (yitU::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAAAAGCTCCTTTAACC, downstream forward: _UP4_TAAAGAAGGTCCGTATTAAT
  • BKK11140 (yitU::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAAAAGCTCCTTTAACC, downstream forward: _UP4_TAAAGAAGGTCCGTATTAAT
  • References

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