spo0B [2018-09-15 11:31:42]

[category|SW 4.2|Sporulation] initiation phosphotransferase of the [SW|phosphorelay]

locus: BSU27930

pI: 4.96

mw: 22.40 kDa

protein length: 192 aa

gene length: 576 bp

function: initiation of [SW|sporulation]

product: [SW|sporulation ]initiation phosphotransferase

essential: no

synonyms: spo0D

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.3|The phosphotransferases]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.3|The phosphotransferases]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

Gene

Coordinates: 2,853,981 → 2,854,559

Phenotypes of a mutant

• Arrest of [SW|sporulation ]at stage 0 (initiation) [pubmed|4633157]

The protein

Structure

• [PDB|1F51] (complex with [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|Spo0F])
• [PDB|1IXM]

Expression and Regulation

Operons

• genes: [gene|29D91115BB3AC03538D618E54A5A57F92777EFF6|spo0B]-[gene|84E7D672DB40B114DC4D96A4D8834958B47401A7|obg]-[gene|A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR]-[gene|D4CCF6727AAC048792E59717E588C36EB617249D|pheA]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2537815], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  additional information

  • expression is repressed by binding of [SW|RpoE] to the A-rich sequence in the -35 region of the promoter [Pubmed|27679485]

Biological materials

Mutant

• [http://www.ncbi.nlm.nih.gov/nuccore/254763670 spo0B12], point mutation 85C>T (=R29W), available in [http://www.bgsc.org/ BGSC] 1S54
• [http://www.ncbi.nlm.nih.gov/nuccore/254763672 spo0B136], amber mutation 103A>T (=K35X), available in [http://www.bgsc.org/ BGSC] 1S16
• [http://www.ncbi.nlm.nih.gov/nuccore/254763674 spo0B580ts], point mutation 193G>A (=E65K), available in [http://www.bgsc.org/ BGSC] 1S90
• [http://www.ncbi.nlm.nih.gov/nuccore/254763676 spo0B581ts], point mutation 461G>A (=G154D), available in [http://www.bgsc.org/ BGSC] 1S91
• BKE27930 (Δ[gene|29D91115BB3AC03538D618E54A5A57F92777EFF6|spo0B]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCGCACTCCCAATCA, downstream forward: _UP4_TAGCGGAGTTTTTAACGGTT
• BKK27930 (Δ[gene|29D91115BB3AC03538D618E54A5A57F92777EFF6|spo0B]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCGCACTCCCAATCA, downstream forward: _UP4_TAGCGGAGTTTTTAACGGTT

References

Reviews

• 28886686

Original Publications

• 20413551,12730135,2437099,9141138,3918016,9299348,16788205,12067336,9726997,2118512,10997904,1846779,9477965,2537815,23490197,27501195