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ptsH [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

HPr, General component of the sugar phosphotransferase system (PTS)

locus

BSU13900

4.00

9.00 kDa

function

PTS-dependent sugar transport and carbon catabolite repression

product

histidine-containing phosphocarrier protein HPr of the PTS

essential

2.7.11.-

synonyms

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

Hide small RNAs Zoom:

categories

[category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.1|General PTS proteins]

[category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]

[category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

[category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

[SW|Categories] containing this gene/protein

[SW|phosphotransferase systems], [SW|transcription factors and their control], [SW|phosphoproteins], [SW|most abundant proteins]

This gene is a member of the following [SW|regulons]

[SW|GlcT regulon], [SW|stringent response]

Gene

Coordinates on the chromosome (coding sequence)

1,459,384 -> 1,459,650

The protein

Catalyzed reaction/ biological activity

Protein HPr N(pi)-phospho-L-histidine protein EIIA = protein HPr protein EIIA N(tau)-phospho-L-histidine (according to Swiss-Prot) Protein HPr N(pi)-phospho-L-histidine protein EIIA = protein [PtsH|search|HPr] protein EIIA N(tau)-phospho-L-histidine

Protein family

HPr domain (according to Swiss-Prot) HPr family

Paralogous protein(s)

[protein|search|Crh]

[SW|Domains]

HPr Domain (2–88)

Modification

transient phosphorylation by [PtsI |search|Enzyme I] of the PTS on His-15

regulatory phosphorylation on Ser-46 by [protein|search|HprK] [Pubmed|2507315]

an extensive study on ''in vivo'' HPr phosphorylation can be found in Singh et al. (2008) [http://www.ncbi.nlm.nih.gov/sites/entrez/18757537 PubMed]

weak phosphorylation on Ser-12 [Pubmed|17218307]

''in vitro'' phosphorylated by [protein|search|PrkC] on Ser-12 [Pubmed|20389117]

Structure

[PDB|2HID] (NMR) [Pubmed|9336834]

[PDB|1KKM] (complex of ''L. casei'' [protein|search|HprK] with ''B. subtilis'' HPr-Ser-P)

[PDB|1KKL] (complex of ''Lactobacillus casei'' [protein|search|HprK] with ''B. subtilis'' HPr)

[PDB|3OQO] (complex of ''B. subtilis'' CcpA with P-Ser-[PtsH|search|HPr] and a optimal synthetic operator site)

[SW|Localization]

cytoplasm [Pubmed|16395550]

[SW|Interactions]

[PtsI|search|Enzyme I]-[PtsH|search|HPr]

[PtsH|search|HPr]-[protein|search|GlpK]

Expression and Regulation

Operon

[SW|Sigma factor]

Regulation

expression activated by glucose (2 fold) ([protein|search|GlcT]) [Pubmed|12850135]

the ''[protein|search|ptsH]'' promoter is constitutive [Pubmed|11902727]

subject to negative stringent control upon amino acid limitation (due to control of'' [protein|search|ptsG]'' transcription initiation) [Pubmed|20081037]

Regulatory mechanism

Additional information

belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Biological materials

Mutant

available in [SW|Jörg Stülke]'s lab:

MZ303 (cat)

GP507 ptsH1 (S46A)

GP506 (ptsH-H15A)

Expression vector

pGP438 (with N-terminal Strep-tag, in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

pAG2 (His-tag) [Pubmed|9237995], available in [SW|Anne Galinier] lab

pGP371(expression / purification of HPr-S46A, with His-tag from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

pGP1415 (HPr, expression in ''B. subtilis'', from [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab

pGP961 (HPr, expression in ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

pGP1416 (HPr-H15A, expression in ''B. subtilis'', from [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab

pGP2431 (N-terminal Strep-tag, expression and purification from ''B. subtilis'', in [SW|pGP380]), for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

two-hybrid system

''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

Antibody

available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

[SW|Josef Deutscher], Paris-Grignon, France

[SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

[SW|Wolfgang Hillen], Erlangen University, Germany [http://www.biologie.uni-erlangen.de/mibi/index2.html Homepage]

[SW|Richard Brennan], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]

[SW|Boris Görke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/goerke.html Homepage]

[SW|Anne Galinier], University of Marseille, France

References

16519689,12850135,17218307,16519689,17142398,12359875,1577686,9162046,11929549,9336834,7803390,7623661,2846556,8169206,9973552,15126459,10048041,12169607,9622354,10217795,17693724,18757537,9202047,7592487,15369672,14527945,2507315,8580838,19651770,8418852,26282429,1303754,1549615,20081037,20389117,20444094,22001508,22722928,23551403,15378759,26381121,9336834