SubtiBank SubtiBank
ptsH [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

ptsH [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

HPr, General component of the sugar phosphotransferase system (PTS)
Locus
BSU13900
Isoelectric point
4.00
Molecular weight
9.00 kDa
Function
PTS-dependent sugar transport and carbon catabolite repression
Product
histidine-containing phosphocarrier protein HPr of the PTS
Essential
no
E.C.
2.7.11.-
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
Loading

Categories containing this gene/protein

  • Cellular processes → Transporters → Phosphotransferase system → General PTS proteins
  • Information processing → Regulation of gene expression → Transcription factors and their control → Control of transcription factor (other than two-component system)
  • Groups of genes → Phosphoproteins → Phosphorylation on a His residue
  • Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

    • Categories containing this gene/protein

  • phosphotransferase systems, transcription factors and their control, phosphoproteins, most abundant proteins

    • This gene is a member of the following regulons

  • GlcT regulon, stringent response

    • Gene

    Coordinates on the chromosome (coding sequence)
    1,459,384 -> 1,459,650

    The protein

    Catalyzed reaction/ biological activity

  • Protein HPr N(pi)-phospho-L-histidine protein EIIA = protein HPr protein EIIA N(tau)-phospho-L-histidine (according to Swiss-Prot) Protein HPr N(pi)-phospho-L-histidine protein EIIA = protein [PtsH|search|HPr] protein EIIA N(tau)-phospho-L-histidine

    • Protein family

  • HPr domain (according to Swiss-Prot) HPr family

    • Paralogous protein(s)

  • Crh

    • Domains

  • HPr Domain (2–88)

    • Modification

  • transient phosphorylation by [PtsI |search|Enzyme I] of the PTS on His-15
  • regulatory phosphorylation on Ser-46 by HprK PubMed
  • an extensive study on in vivo HPr phosphorylation can be found in Singh et al. (2008) PubMed
  • weak phosphorylation on Ser-12 PubMed
  • in vitro phosphorylated by PrkC on Ser-12 PubMed

    • Structure

  • 2HID (NMR) PubMed
  • 1KKM (complex of L. casei HprK with B. subtilis HPr-Ser-P)
  • 1KKL (complex of Lactobacillus casei HprK with B. subtilis HPr)
  • 3OQM (complex of B. subtilis CcpA with P-Ser-[PtsH|search|HPr] and the ackA operator site)
  • 3OQN (complex of B. subtilis CcpA with P-Ser-[PtsH|search|HPr] and the gntR operator site)
  • 3OQO (complex of B. subtilis CcpA with P-Ser-[PtsH|search|HPr] and a optimal synthetic operator site)

    • Localization

  • cytoplasm PubMed

    • Interactions

  • HprK-[PtsH|search|HPr] PubMed
  • [PtsI|search|Enzyme I]-[PtsH|search|HPr]
  • [PtsH|search|HPr]-PtsG PubMed, PtsH-MtlF PubMed, ManP-[PtsH|search|HPr], GmuA-[PtsH|search|HPr]
  • GamP-[PtsH|search|HPr], BglP-[PtsH|search|HPr], LicA-[PtsH|search|HPr], LevD-[PtsH|search|HPr]
  • FruA-[PtsH|search|HPr], YpqE-[PtsH|search|HPr]
  • [PtsH|search|HPr]-LicT, [PtsH|search|HPr]-SacY, [PtsH|search|HPr]-SacT, [PtsH|search|HPr]-GlcT
  • [PtsH|search|HPr]-MtlR PubMed, [PtsH|search|HPr]-LicR, [PtsH|search|HPr]-LevR,[PtsH|search|HPr]-ManR
  • [PtsH|search|HPr]-GlpK
  • GapA-[PtsH|search|HPr] PubMed
  • [PtsH|search|HPr(Ser-46)-P]-CcpA PubMed
  • [PtsH|search|HPr]-RbsR PubMed
  • [PtsH|search|HPr(His)-P]-RhgR PubMed

    • Expression and Regulation

    Operon

  • ptsG-ptsH-ptsI PubMed
  • ptsH-ptsI PubMed

    • Sigma factor

  • ptsG: SigA PubMed
  • ptsH: SigA PubMed

    • Regulation

  • expression activated by glucose (2 fold) (GlcT) PubMed
  • the ptsH promoter is constitutive PubMed
  • subject to negative stringent control upon amino acid limitation (due to control of ptsG transcription initiation) PubMed

    • Regulatory mechanism

  • ptsG: transcriptional antitermination via the GlcT-dependent RNA switch PubMed

    • Additional information

  • belongs to the 100 most abundant proteins PubMed

    • Biological materials

    Mutant

  • available in Jörg Stülke's lab:
  • MZ303 (cat)
  • GP507 ptsH1 (S46A)
  • GP506 (ptsH-H15A)
  • GP778 (replacement of glcT and the ptsG-ptsH-ptsI operon by a spc cassette), PubMed

    • Expression vector

  • pGP438 (with N-terminal Strep-tag, in pGP172), available in Jörg Stülke's lab
  • pAG2 (His-tag) PubMed, available in Anne Galinier lab
  • pGP371(expression / purification of HPr-S46A, with His-tag from E. coli, in pWH844), available in Jörg Stülke's lab
  • pGP1415 (HPr, expression in B. subtilis, from pBQ200), available in Jörg Stülke's lab
  • pGP961 (HPr, expression in B. subtilis with N-terminal Strep-tag, for SPINE, available in Jörg Stülke's lab
  • pGP1416 (HPr-H15A, expression in B. subtilis, from pBQ200), available in Jörg Stülke's lab
  • pGP2431 (N-terminal Strep-tag, expression and purification from B. subtilis, in pGP380), for SPINE, available in Jörg Stülke's lab

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • Antibody

  • available in Jörg Stülke's lab

    • Labs working on this gene/protein

  • Josef Deutscher, Paris-Grignon, France
  • Jörg Stülke, University of Göttingen, Germany Homepage
  • Wolfgang Hillen, Erlangen University, Germany Homepage
  • Richard Brennan, Houston, Texas, USA Homepage
  • Boris Görke, University of Göttingen, Germany Homepage
  • Anne Galinier, University of Marseille, France

    • References

    Loading