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ptsH [Fri Sep 23 2016 20:01:16 GMT+0200 (CEST)]
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ptsH [Fri Sep 23 2016 20:01:16 GMT+0200 (CEST)]

HPr, General component of the sugar phosphotransferase system (PTS)
locus
BSU13900
pI
4.00
mw
9.00 kDa
function
PTS-dependent sugar transport and carbon catabolite repression
product
histidine-containing phosphocarrier protein HPr of the PTS
essential
no
ec
2.7.11.-
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.1|General PTS proteins]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

    • [SW|Categories] containing this gene/protein

  • [SW|phosphotransferase systems], [SW|transcription factors and their control], [SW|phosphoproteins], [SW|most abundant proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|GlcT regulon], [SW|stringent response]

    • Gene

    Coordinates on the chromosome (coding sequence)
    1,459,384 -> 1,459,650

    The protein

    Catalyzed reaction/ biological activity

  • Protein HPr N(pi)-phospho-L-histidine protein EIIA = protein HPr protein EIIA N(tau)-phospho-L-histidine (according to Swiss-Prot) Protein HPr N(pi)-phospho-L-histidine protein EIIA = protein [PtsH|search|HPr] protein EIIA N(tau)-phospho-L-histidine

    • Protein family

  • HPr domain (according to Swiss-Prot) HPr family

    • Paralogous protein(s)

  • [protein|search|Crh]

    • [SW|Domains]

  • HPr Domain (2–88)

    • Modification

  • transient phosphorylation by [PtsI |search|Enzyme I] of the PTS on His-15
  • regulatory phosphorylation on Ser-46 by [protein|search|HprK] [Pubmed|2507315]
  • an extensive study on ''in vivo'' HPr phosphorylation can be found in Singh et al. (2008) [http://www.ncbi.nlm.nih.gov/sites/entrez/18757537 PubMed]
  • weak phosphorylation on Ser-12 [Pubmed|17218307]
  • ''in vitro'' phosphorylated by [protein|search|PrkC] on Ser-12 [Pubmed|20389117]

    • Structure

  • [PDB|2HID] (NMR) [Pubmed|9336834]
  • [PDB|1KKM] (complex of ''L. casei'' [protein|search|HprK] with ''B. subtilis'' HPr-Ser-P)
  • [PDB|1KKL] (complex of ''Lactobacillus casei'' [protein|search|HprK] with ''B. subtilis'' HPr)
  • [PDB|3OQM] (complex of ''B. subtilis'' CcpA with P-Ser-[PtsH|search|HPr] and the ''[protein|search|ackA]'' operator site)
  • [PDB|3OQN] (complex of ''B. subtilis'' CcpA with P-Ser-[PtsH|search|HPr] and the ''[protein|search|gntR]'' operator site)
  • [PDB|3OQO] (complex of ''B. subtilis'' CcpA with P-Ser-[PtsH|search|HPr] and a optimal synthetic operator site)

    • [SW|Localization]

  • cytoplasm [Pubmed|16395550]

    • [SW|Interactions]

  • [protein|search|HprK]-[PtsH|search|HPr] [Pubmed|12009882]
  • [PtsI|search|Enzyme I]-[PtsH|search|HPr]
  • [PtsH|search|HPr]-[protein|search|PtsG] [Pubmed|8418852], [protein|search|PtsH]-[protein|search|MtlF] [Pubmed|20444094], [protein|search|ManP]-[PtsH|search|HPr], [protein|search|GmuA]-[PtsH|search|HPr]
  • [protein|search|GamP]-[PtsH|search|HPr], [protein|search|BglP]-[PtsH|search|HPr], [protein|search|LicA]-[PtsH|search|HPr], [protein|search|LevD]-[PtsH|search|HPr]
  • [protein|search|FruA]-[PtsH|search|HPr], [protein|search|YpqE]-[PtsH|search|HPr]
  • [PtsH|search|HPr]-[protein|search|LicT], [PtsH|search|HPr]-[protein|search|SacY], [PtsH|search|HPr]-[protein|search|SacT], [PtsH|search|HPr]-[protein|search|GlcT]
  • [PtsH|search|HPr]-[protein|search|MtlR] [Pubmed|20444094], [PtsH|search|HPr]-[protein|search|LicR], [PtsH|search|HPr]-[protein|search|LevR],[PtsH|search|HPr]-[protein|search|ManR]
  • [PtsH|search|HPr]-[protein|search|GlpK]
  • [protein|search|GapA]-[PtsH|search|HPr] [Pubmed|17142398]
  • [PtsH|search|HPr(Ser-46)-P]-[protein|search|CcpA] [Pubmed|12432959]
  • [PtsH|search|HPr]-[protein|search|RbsR] [Pubmed|16519689]
  • [PtsH|search|HPr(His)-P]-[protein|search|RhgR] [Pubmed|19651770]

    • Expression and Regulation

    Operon

  • ''[protein|search|ptsG]-[protein|search|ptsH]-[protein|search|ptsI]'' [Pubmed|11902727]
  • ''[protein|search|ptsH]-[protein|search|ptsI]'' [Pubmed|11902727]

    • [SW|Sigma factor]

  • ''[protein|search|ptsG]'': [protein|search|SigA] [Pubmed|11902727]
  • ''[protein|search|ptsH]'': [protein|search|SigA] [Pubmed|11902727]

    • Regulation

  • expression activated by glucose (2 fold) ([protein|search|GlcT]) [Pubmed|12850135]
  • the ''[protein|search|ptsH]'' promoter is constitutive [Pubmed|11902727]
  • subject to negative stringent control upon amino acid limitation (due to control of'' [protein|search|ptsG]'' transcription initiation) [Pubmed|20081037]

    • Regulatory mechanism

  • ''[protein|search|ptsG]'': transcriptional antitermination via the [protein|search|GlcT]-dependent [SW|RNA switch] [Pubmed|9765562]

    • Additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Biological materials

    Mutant

  • available in [SW|Jörg Stülke]'s lab:
  • MZ303 (cat)
  • GP507 ptsH1 (S46A)
  • GP506 (ptsH-H15A)
  • GP778 (replacement of ''[protein|search|glcT]'' and the ''[protein|search|ptsG]-[protein|search|ptsH]-[protein|search|ptsI]'' operon by a spc cassette), [Pubmed|22722928]

    • Expression vector

  • pGP438 (with N-terminal Strep-tag, in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
  • pAG2 (His-tag) [Pubmed|9237995], available in [SW|Anne Galinier] lab
  • pGP371(expression / purification of HPr-S46A, with His-tag from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP1415 (HPr, expression in ''B. subtilis'', from [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
  • pGP961 (HPr, expression in ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
  • pGP1416 (HPr-H15A, expression in ''B. subtilis'', from [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
  • pGP2431 (N-terminal Strep-tag, expression and purification from ''B. subtilis'', in [SW|pGP380]), for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • Antibody

  • available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Josef Deutscher], Paris-Grignon, France
  • [SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
  • [SW|Richard Brennan], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]
  • [SW|Boris Görke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/goerke.html Homepage]
  • [SW|Anne Galinier], University of Marseille, France

    • References

  • 16519689,12850135,17218307,16519689,17142398,12359875,1577686,9162046,11929549,9336834,7803390,7623661,2846556,8169206,9973552,15126459,10048041,12169607,9622354,10217795,17693724,18757537,9202047,7592487,15369672,14527945,2507315,8580838,19651770,8418852,26282429,1303754,1549615,20081037,20389117,20444094,22001508,22722928,23551403,15378759,26381121,9336834