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ytlP [2019-08-22 10:17:15]
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ytlP [2019-08-22 10:17:15]

putative 2-5 RNA-ligase
locus
BSU_29960
pI
8.50
mw
21.00 kDa
protein length
183 aa Sequence Blast
gene length
552 bp Sequence Blast
function
RNA metabolism
product
putative 2-5 RNA-ligase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.4|Nucleotide metabolism/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    3,065,751 3,066,302

    The protein

    Catalyzed reaction/ biological activity

  • H2O + RNA(n)-2',3'-cyclic phosphate --> H+ + RNA(n)-2'- phosphate (according to UniProt)

    • Protein family

  • 2H phosphoesterase superfamily (with [protein|5CA17CD28A725B8D7B87B9AAA167844042439794|YjcG], according to UniProt)

    • Paralogous protein(s)

  • [protein|5CA17CD28A725B8D7B87B9AAA167844042439794|YjcG]

    • Structure

  • [PDB|1IUH] (from Thermus thermophilus, 24% identity) [pubmed|12798681]

    • Expression and Regulation

    Operons

    genes
    [gene|29A38D244A07ED0973D0846B652238524F4DBC67|ytlP]-[gene|DB1491F13F8B55A360413E815E47A2B51D24395C|ytlQ]-[gene|ED4C1EA16FF96DA20F12B972CD2458FA3B49DDA5|ytlR]-[gene|7D783DD180D950A4B030BF5EA78CCE930A466A07|amyX]
    description
    [pubmed|22383849]

    sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|26577401], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]
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    Biological materials

    Mutant

  • MGNA-A431 (ytlP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/431 NBRP B. subtilis, Japan]
  • BKE29960 ([gene|29A38D244A07ED0973D0846B652238524F4DBC67|ytlP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGTCTGACACTCCCTT, downstream forward: _UP4_TAATTCATTGCGGAGTGGAG
  • BKK29960 ([gene|29A38D244A07ED0973D0846B652238524F4DBC67|ytlP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGTCTGACACTCCCTT, downstream forward: _UP4_TAATTCATTGCGGAGTGGAG

    • References

  • 17012785,12798681