clpX

AAA unfoldase, ATP-dependent Clp protease, ATP-binding subunit (class III heat-shock protein)

locus: BSU_28220

pI: 4.64

mw: 46.25 kDa

protein length: 420 aa

gene length: 1263 bp

function: protein degradation

product: AAA unfoldase, ATP-dependent Clp protease, ATP-binding subunit

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]

Gene

Coordinates: 2,884,781 2,886,043

Phenotypes of a mutant

• increased thermotolerance due to increased stabiliy of [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx] and thus increased expression of ''[gene|4E5C84FDC8FE2FEFF47306C91ECAB7F17D3E38E9|trxA]'' [Pubmed|24417481]
• the mutation suppresses the heat sensitivity of spores overexpressing ''[gene|85DE3CB6CDA61C141C6030367C0A13BB642160B9|cmpA]'' [Pubmed|26387458]

The protein

Catalyzed reaction/ biological activity

• ATPase/chaperone

Protein family

• ClpX chaperone family (with [protein|2A5A080273CB7698DFABB147F4143E90BBCA3B01|ClpY], according to UniProt)

[SW|Domains]

• AAA-ATPase [http://pfam.sanger.ac.uk/family?acc=PF07724 PFAM]
• Zinc finger [http://pfam.sanger.ac.uk/family?acc=PF06689 PFAM]

Structure

• [PDB|1UM8] (from ''Helicobacter pylori'') [Pubmed|14514695]

[SW|Localization]

• cytoplasmic polar clusters, excluded from the nucleoid, induced clustering upon heat shock, colocalization with [protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|ClpP] [Pubmed|18786145,18689473]

Expression and Regulation

Operons

• genes: [gene|297F53DAD3351E0C55108DD2C93B78FFB174438C|clpX]
  description: [Pubmed|11325926]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8973311], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|908DB17A39D518E84977250C55825E77FA02E391|CtsR]: repression, [Pubmed|9852015], in [regulon|908DB17A39D518E84977250C55825E77FA02E391|CtsR regulon]

  regulation

  • induced by heat stress ([protein|search|CtsR]) [Pubmed|9852015]

Biological materials

Mutant

• MGNA-B019 (clpX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1018 NBRP B. subtilis, Japan]
• GPUG2 (aphA3), available in [SW|Ulf Gerth]'s and [SW|Jörg Stülke]'s labs
• GP1787 (aphA3), available in [SW|Jörg Stülke]'s lab
• ''clpX::kan'', ''clpX::spec'' and ''clpX::cat'' available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen]] Lab
• BKE28220 ([gene|297F53DAD3351E0C55108DD2C93B78FFB174438C|clpX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTTCACCCCTTAATC, downstream forward: _UP4_TAAAGATAAGCACAAACCTC
• BKK28220 ([gene|297F53DAD3351E0C55108DD2C93B78FFB174438C|clpX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTTCACCCCTTAATC, downstream forward: _UP4_TAAAGATAAGCACAAACCTC

GFP fusion

• C-terminal GFP fusions (both single copy and 2th copy in ''amyE'' locus, also as CFP and YFP variants) available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen]] Lab

labs

• [SW|Leendert Hamoen], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen homepage]

References

Reviews

• 23375660,19680248,17302811,23479438,19609260,26639779,28748186

Original Publications

• 12761164,10809708,9643546,11807061,14679237,18689476,16899079,8973311,19136590,11325926,8973311,9852015,18689473,20525796,15948963,18786145,24417481,24942655,25433860,25866879,14514695,26387458,27669037,29625553,32477307