SubtiBank SubtiBank
fusA [Wed Sep 21 2016 12:01:37 GMT+0200 (CEST)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

fusA [Wed Sep 21 2016 12:01:37 GMT+0200 (CEST)]

elongation factor G, facilitates movement of tRNA–mRNA by one codon
locus
BSU01120
pI
4.00
mw
76.00 kDa
function
translation
product
elongation factor G
essential
yes
synonyms
fus
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.11|Translation factors]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue]

    • [SW|Categories] containing this gene/protein

  • [SW|translation], [SW|essential genes], [SW|phosphoproteins], [SW|most abundant proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|stringent response]

    • Gene

    Coordinates on the chromosome (coding sequence)
    130,684 -> 132,762

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • hydrolyses GTP

    • Protein family

  • EF-G/EF-2 subfamily (according to Swiss-Prot)

    • Modification

  • phosphorylation on Ser-213 AND Ser-302 AND Ser-569 AND Ser-680 AND (Thr-24 OR Thr-25) AND (Thr-43 OR Ser 48) [Pubmed|17218307], [Pubmed|17726680]
  • phosphorylated on Tyr-339 [Pubmed|26381121]

    • Structure

  • [PDB|1ELO] (from ''Thermus thermophilus'') [Pubmed|8070397]
  • [PDB|4BTC], [PDB|4BTD], the ''Thermus thermophilus'' EF-G–[SW|ribosome] complex in a pretranslocation state [Pubmed|23912278]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • [SW|Interactions]

  • [protein|search|FusA]-[protein|search|RplL] [Pubmed|23912278]
  • [protein|search|FusA]-[protein|search|RpsL] [Pubmed|23912278]

    • Expression and Regulation

    Regulation

  • [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

    • Additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Biological materials

    Expression vector

  • for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP840, available in [SW|Jörg Stülke]'s lab
  • for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP848, available in [SW|Jörg Stülke]'s lab

    • References

  • 24614227,17726680,17218307,20348921,8070397,23912278,15378759,25108354,26381121