SubtiBank SubtiBank
fusA
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fusA

elongation factor G, facilitates movement of tRNA and mRNA by one codon
Locus
BSU_01120
Isoelectric point
4.61
Molecular weight
76.36 kDa
Protein length
692 aa Sequence Blast
Gene length
2079 bp Sequence Blast
Function
translation
Product
elongation factor G
Essential
yes
Synonyms
fus
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → Translation factors
  • Groups of genes → Essential genes
  • Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue
  • Groups of genes → Phosphoproteins → Phosphorylation on a Thr residue
  • Groups of genes → Phosphoproteins → Phosphorylation on a Tyr residue

    • Gene

    Coordinates
    130,684 132,762

    Phenotypes of a mutant

  • essential PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • hydrolyses GTP

    • Protein family

  • TRAFAC class translation factor GTPase superfamily (according to UniProt)
  • Classic translation factor GTPase family (according to UniProt)

    • Domains

  • tr-type G domain (aa 8-282) (according to UniProt)

    • Modification

  • phosphorylation on Ser-213 AND Ser-302 AND Ser-569 AND Ser-680 AND (Thr-24 OR Thr-25) AND (Thr-43 OR Ser 48) PubMed, PubMed
  • phosphorylated on Tyr-339 PubMed

    • Structure

  • 1ELO (from Thermus thermophilus) PubMed
  • 4BTC, 4BTD, the Thermus thermophilus EF-Gribosome complex in a pretranslocation state PubMed

    • Localization

  • cytoplasm (according to UniProt)

    • Additional information

  • belongs to the 100 most abundant proteins PubMed

    • Expression and Regulation

    Operons

    Genes
    rplGB-rpsL-rpsG-fusA-tufA
    Description
    PubMed

    Regulatory mechanism

  • stringent response: negative regulation, PubMed, in stringent response

    • Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed
  • the mRNA is processed between rplGB and rpsL by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed
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    Biological materials

    Expression vectors

  • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP840, available in Jörg Stülke's lab
  • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP848, available in Jörg Stülke's lab

    • References

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