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rhaM [2019-01-30 19:23:43]
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rhaM [2019-01-30 19:23:43]

mutarotase involved in rhamnose utilization
Locus
BSU31190
Isoelectric point
5.18
Molecular weight
12.44 kDa
Protein length
104 aa Sequence Blast
Gene length
315 bp Sequence Blast
Function
utilization of rhamnose
Product
mutarotase
Essential
no
Synonyms
yulD

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,199,233 3,199,547

The protein

Catalyzed reaction/ biological activity

  • Alpha-L-rhamnose = beta-L-rhamnose (according to Swiss-Prot)
  • Protein family

  • rhamnose mutarotase family (according to Swiss-Prot)
  • Structure

  • 1X8D (from E. coli, 55% identity)
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • RhaR: repression, PubMed, in RhaR regulon
  • CcpA: repression, PubMed, in CcpA regulon
  • Regulation

  • expression during spore germination is increased under conditions of osmotic stress PubMed
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    Description

    Regulation

  • expression during spore germination is increased under conditions of osmotic stress PubMed
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    Biological materials

    Mutant

  • MGNA-A639 (yulD::erm), available at the NBRP B. subtilis, Japan
  • BKE31190 (rhaM::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAACCTTTTCACCTCTTCA, downstream forward: _UP4_TGAAAAACTGATAAAGGGAG
  • BKK31190 (rhaM::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAACCTTTTCACCTCTTCA, downstream forward: _UP4_TGAAAAACTGATAAAGGGAG
  • References

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