rhaM [2019-01-30 19:23:43]

mutarotase involved in rhamnose utilization

Locus: BSU31190

Isoelectric point: 5.18

Molecular weight: 12.44 kDa

Protein length: 104 aa

Gene length: 315 bp

Function: utilization of rhamnose

Product: mutarotase

Essential: no

Synonyms: yulD

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of rhamnose

Gene

Coordinates: 3,199,233 3,199,547

The protein

Catalyzed reaction/ biological activity

• Alpha-L-rhamnose = beta-L-rhamnose (according to Swiss-Prot)

Protein family

• rhamnose mutarotase family (according to Swiss-Prot)

Structure

• 1X8D (from E. coli, 55% identity)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: rhaEW-rhaR-rhaB-rhaM-rhaA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • RhaR: repression, PubMed, in RhaR regulon
  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • expression during spore germination is increased under conditions of osmotic stress PubMed

• Genes: rhaR-rhaB-rhaM-rhaA
  Description: PubMed

  Regulation

  • expression during spore germination is increased under conditions of osmotic stress PubMed

Biological materials

Mutant

• MGNA-A639 (yulD::erm), available at the NBRP B. subtilis, Japan
• BKE31190 (rhaM::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAACCTTTTCACCTCTTCA, downstream forward: _UP4_TGAAAAACTGATAAAGGGAG
• BKK31190 (rhaM::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAACCTTTTCACCTCTTCA, downstream forward: _UP4_TGAAAAACTGATAAAGGGAG

References

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