ywbG

holin-like auxilary protein

Locus: BSU_38330

Isoelectric point: 9.56

Molecular weight: 25.67 kDa

Protein length: 225 aa

Gene length: 678 bp

Function: unknown

Product: holin-like auxilary protein

Essential: no

Synonyms: ipa-22r

Genomic Context

Loading

Categories containing this gene/protein

• Groups of genes → Membrane proteins
• Groups of genes → Proteins of unknown function

Gene

Coordinates: 3,933,577 3,934,254

Phenotypes of a mutant

• a ywbH-ywbG mutant is delayed in pellicle formation PubMed
• a ywbH-ywbG yxaK-yxaC pftA-pftB triple mutant is severely delayed in pellicle formation PubMed
• a ywbH-ywbG overexpression strain has more robust pellicle formation PubMed

The protein

Protein family

• YohK (E.coli)/YwbG (IPA-22R) (B.subtilis) family (with YxaC, according to UniProt)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: ywbH-ywbG
  Description: PubMed

  Regulatory mechanism

  • YwbI: activation, PubMed, in YwbI regulon

  Regulation

  • induced by acetate (YwbI) PubMed

Biological materials

Mutant

• MGNA-B224 (ywbG::erm), available at the NBRP B. subtilis, Japan
• BKE38330 (ywbG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACTTACAATTCCGATGAACA, downstream forward: _UP4_TAAAAAACTGTCCTCTCGAG
• BKK38330 (ywbG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACTTACAATTCCGATGAACA, downstream forward: _UP4_TAAAAAACTGTCCTCTCGAG

References

Loading