ydaG [2018-06-05 17:15:33]

general stress protein, required for protection against paraquat stress

locus: BSU04220

pI: 5.20

mw: 15.00 kDa

protein length: 140 aa

gene length: 420 bp

function: survival of stress conditions

product: unknown

essential: no

synonyms: yzzA

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 473,803 → 474,225

Expression and Regulation

Operons

• genes: [gene|BFDAB855DC76A78C100DC5E5FB81F73069F5F93F|ydaF]-[gene|29342783A375260C5DAE920FBF6EC6EFD2D54DDE|ydaG]
  description: [Pubmed|22383849]

• genes: [gene|29342783A375260C5DAE920FBF6EC6EFD2D54DDE|ydaG]
  description: [Pubmed|10220166]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|10220166], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • induced under stress conditions ([protein|search|SigB]) [Pubmed|10220166]

• genes: [gene|3161519994609DA9360ED6E073E4A4C8C6210EFB|ydaD]-[gene|DDE975DA2E13759B0DB8B6D219760C2E2C6032FB|ydaE]-[gene|BFDAB855DC76A78C100DC5E5FB81F73069F5F93F|ydaF]-[gene|29342783A375260C5DAE920FBF6EC6EFD2D54DDE|ydaG]
  description: [Pubmed|10220166]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528,10220166], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|15805528]

Biological materials

Mutant

• MGNA-C102 (ydaG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2100 NBRP B. subtilis, Japan]
• BKE04220 (Δ[gene|29342783A375260C5DAE920FBF6EC6EFD2D54DDE|ydaG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCGAATCACTCCTTTT, downstream forward: _UP4_TAAAAAATTTGTGTTTTCAG
• BKK04220 (Δ[gene|29342783A375260C5DAE920FBF6EC6EFD2D54DDE|ydaG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCGAATCACTCCTTTT, downstream forward: _UP4_TAAAAAATTTGTGTTTTCAG

References

• 10220166,22582280,15805528,23033921