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sspB [2017-8-11 16:0:3]
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sspB [2017-8-11 16:0:3]

small acid-soluble spore protein (major beta-type SASP)
locus
BSU09750
pI
4.79
mw
6.84 kDa
protein length
67 aa Sequence Blast
gene length
201 bp Sequence Blast
function
protection of spore DNA
product
small acid-soluble spore protein (major beta-type SASP)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.3|Small acid-soluble spore proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

    • Gene

    Coordinates
    1,050,031 → 1,050,234

    Phenotypes of a mutant

  • a ''[gene|28F47ADECD376494878AE58B395B3A2616B6B5DF|sspB]'' mutant is sensitive to ionizing radiation [Pubmed|24123749]

    • The protein

    Protein family

  • alpha/beta-type SASP family (according to Swiss-Prot)

    • Modification

  • phosphorylated on Ser-6, Ser-7, and Ser-45 [Pubmed|26381121]

    • Structure

  • [PDB|2Z3X] ([protein|9E1B3A6289F2C94F9088FA14CE8939E208DE9FEA|SspC] in complex with DNA, 78% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|28F47ADECD376494878AE58B395B3A2616B6B5DF|sspB]
    description
    [Pubmed|3009398]

    sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|15699190], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

    • regulatory mechanism

  • [protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT]: activation, [Pubmed|8755877], in [regulon|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT regulon]

    • regulation

  • expressed during sporulation in the forespore ([protein|search|SigG], [SW|SpoVT]) [Pubmed|15699190,8755877]

    • additional information

  • the mRNA half-life is about 13 min [PubMed|24163345]
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    Biological materials

    Mutant

  • 1S111 (no resistance), [Pubmed|3087950], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S111&Search=1S111 BGSC]
  • 1S113 (no resistance), [Pubmed|3125155], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S113&Search=1S113 BGSC]

    • GFP fusion

  • GP996 (cat, based on pSG1151), available in the [SW|Stülke] lab

    • References

  • 16707666,1900507,2456528,3009398,11092849,7608092,15458366,19542328,8755877,23064347,3087950,3125155,24163345,24123749,26381121