ctaF [2019-08-01 09:07:24]

cytochrome-c oxidase (subunit IV)

Locus: BSU_14920

Isoelectric point: 9.88

Molecular weight: 12.48 kDa

Protein length: 110 aa

Gene length: 333 bp

Function: respiration

Product: cytochrome-c oxidase (subunit IV)

Essential: no

E.C.: 1.9.3.1

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Electron transport and ATP synthesis → Respiration → Terminal oxidases
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,564,063 1,564,395

The protein

Catalyzed reaction/ biological activity

• 4 ferrocytochrome c + O₂ + 4 H⁺ = 4 ferricytochrome c + 2 H₂O (according to Swiss-Prot)

Protein family

• cytochrome c oxidase bacterial subunit 4 family (with QoxD, according to UniProt)

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ctaB-ctaC-ctaD-ctaE-ctaF-ctaG
  Description: PubMed

  Regulatory mechanism

  • ResD: activation, PubMed, in ResD regulon
  • Abh: repression, synergistic repression with AbrB, in Abh regulon
  • CcpA: repression, in CcpA regulon
  • AbrB: repression, synergistic repression with Abh, in AbrB regulon

  Regulation

  • expressed under anaerobic conditions (ResD) PubMed

Biological materials

Mutant

• BKE14920 (ctaF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCTGCTTTTTTTATCGACCA, downstream forward: _UP4_TAATTTGAACAGCCGCCGGA
• BKK14920 (ctaF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCTGCTTTTTTTATCGACCA, downstream forward: _UP4_TAATTTGAACAGCCGCCGGA

References

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