mneP

primary manganese (II) efflux pump

locus: BSU_05470

pI: 6.16

mw: 32.64 kDa

protein length: 297 aa

gene length: 894 bp

function: Mn(II) export

product: manganese (II) efflux pump

essential: no

synonyms: ydfM

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.13|Metal ion exporters]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.2|Trace metal homeostasis (Cu, Zn, Ni, Mn, Mo)] → [category|SW 1.3.2.2|Manganese]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 595,109 596,002

Phenotypes of a mutant

• sensitive to Mn(II) [Pubmed|27748968]
• a [gene|28C7CFE5701F6920652BE1973DC7A15D7C088E35|mneP] [protein|72BF9B934CB5800E156263FF377DED0AD2E920A9|MneS] double mutant is extremely senitive to Mn²⁺ intoxication [pubmed|31685536]

The protein

Catalyzed reaction/ biological activity

• efflux of Mn(II) [Pubmed|27748968]

Protein family

• [SW|Cation diffusion facilitator (CDF) transporter (TC 2.A.4) family] [Pubmed|27748968]

Paralogous protein(s)

• [protein|72BF9B934CB5800E156263FF377DED0AD2E920A9|MneS], [protein|D52111E81E9233DABAEFCAF6217145CF6A7F3961|YdbO]

Structure

• [PDB|5VRF] (Shewanella oneidensis zinc transporter, 26% identity) [pubmed|29507252]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|28C7CFE5701F6920652BE1973DC7A15D7C088E35|mneP]
  description: [pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|27748968], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|MntR]: transcription activation, [Pubmed|27748968], in [regulon|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|MntR regulon]

  regulation

  • expressed at elevated Mn(II) concentrations ([protein|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|MntR]) [Pubmed|27748968]

Biological materials

Mutant

• MGNA-C152 (ydfM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2150 NBRP B. subtilis, Japan]
• BKE05470 ([gene|28C7CFE5701F6920652BE1973DC7A15D7C088E35|mneP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTCCTCCTATAAAA, downstream forward: _UP4_TGAAAAGCTCCTTTTTAGAA
• BKK05470 ([gene|28C7CFE5701F6920652BE1973DC7A15D7C088E35|mneP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTCCTCCTATAAAA, downstream forward: _UP4_TGAAAAGCTCCTTTTTAGAA

References

• 27748968,29507252,31685536