mneP

primary manganese (II) efflux pump

Locus: BSU_05470

Isoelectric point: 6.16

Molecular weight: 32.64 kDa

Protein length: 297 aa

Gene length: 894 bp

Function: Mn(II) export

Product: manganese (II) efflux pump

Essential: no

Synonyms: ydfM

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Metal ion exporters
• Cellular processes → Homeostasis → Trace metal homeostasis (Cu, Zn, Ni, Mn, Mo) → Manganese
• Groups of genes → Membrane proteins

Gene

Coordinates: 595,109 596,002

Phenotypes of a mutant

• sensitive to Mn(II) PubMed
• a mneP MneS double mutant is extremely senitive to Mn²⁺ intoxication PubMed

The protein

Catalyzed reaction/ biological activity

• efflux of Mn(II) PubMed

Protein family

• Cation diffusion facilitator (CDF) transporter (TC 2.A.4) family PubMed

Paralogous protein(s)

• MneS, YdbO

Structure

• 5VRF (Shewanella oneidensis zinc transporter, 26% identity) PubMed

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: mneP
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • MntR: transcription activation, PubMed, in MntR regulon

  Regulation

  • expressed at elevated Mn(II) concentrations (MntR) PubMed

Biological materials

Mutant

• MGNA-C152 (ydfM::erm), available at the NBRP B. subtilis, Japan
• BKE05470 (mneP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTTCCTCCTATAAAA, downstream forward: _UP4_TGAAAAGCTCCTTTTTAGAA
• BKK05470 (mneP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTTCCTCCTATAAAA, downstream forward: _UP4_TGAAAAGCTCCTTTTTAGAA

References

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