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yabJ [2017-08-16 10:06:52]
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yabJ [2017-08-16 10:06:52]

2-iminobutanoate/2-iminopropanoate deaminase, detoxification of reactive intermediates generated in isoleucine biosynthesis
Locus
BSU00480
Isoelectric point
5.13
Molecular weight
13.51 kDa
Protein length
125 aa Sequence Blast
Gene length
375 bp Sequence Blast
Function
biosynthesis of isoleucine
Product
2-iminobutanoate/2-iminopropanoate deaminase
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of branched-chain amino acids
  • Metabolism → Detoxification reactions

    • Gene

    Coordinates
    55,295 → 55,672

    The protein

    Catalyzed reaction/ biological activity

  • release of ammonia from reactive enamine/imine intermediates of the pyridoxal 5'-phosphate-dependent threonine dehydratase (IlvA) PubMed
  • 2-iminobutanoate + H2O = 2-oxobutanoate + NH3 PubMed
  • 2-iminopropanoate + H2O = pyruvate + NH3 PubMed

    • Protein family

  • Ref.3 (according to Swiss-Prot) YjgF family

    • Structure

  • 1QD9, 1QD9 NCBI PubMed

    • Localization

  • cytoplasm (according to Swiss-Prot)

    • Additional information

  • was originally thought to be required for activity of PurR, but this is due to the orientation of the marker gene downstream of purR in the yabJ mutant strain used in PubMed

    • Expression and Regulation

    Operons

    Genes
    ispE-purR-yabJ
    Description
    PubMed

    Regulatory mechanism

  • PurR: repression, PubMed, in PurR regulon

    • Regulation

  • negative autoregulation PubMed
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    Biological materials

    Mutant

  • MGNA-B911 (yabJ::erm), available at the NBRP B. subtilis, Japan

    • References

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