yokF [2019-09-17 11:58:10]

similar to micrococcal nuclease

locus: BSU_21610

pI: 8.40

mw: 32.85 kDa

protein length: 296 aa

gene length: 891 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.2|SP-beta prophage]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 2,279,977 2,280,867

The protein

Paralogous protein(s)

• [protein|91E6B4971F22B5A5F964590F8B14D6C73A4C4840|YncB]

Structure

• [PDB|4I65] (from Staphylococcus aureus, corresponds to aa 71 ... 198 of YncB, 57% identity)

Expression and Regulation

Operons

• genes: [gene|27F32FB0F96276325ED93710DF77B949658D728D|yokF]-[gene|C4C5A7CCD4B02EC846A40C52574A1092D5A95F68|yokE]
  description: [pubmed|22383849]

Biological materials

Mutant

• BKE21610 ([gene|27F32FB0F96276325ED93710DF77B949658D728D|yokF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE21610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTTCATTTCCCCTTTTA, downstream forward: _UP4_TGTGAACGATGAAAGGAGAT
• BKK21610 ([gene|27F32FB0F96276325ED93710DF77B949658D728D|yokF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK21610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTTCATTTCCCCTTTTA, downstream forward: _UP4_TGTGAACGATGAAAGGAGAT