bkdB [Sat Apr 16 2016 18:03:53 GMT+0200 (CEST)]

2-oxoisovalerate dehydrogenase (E2 subunit, lipoamide acyltransferase)

locus: BSU24030

pI: 5.00

mw: 45.00 kDa

function: utilization of branched-chain keto acids

product: 2-oxoisovalerate dehydrogenase
(E2 subunit, lipoamide acyltransferase)

essential: no

ec: 2.3.1.168

synonyms: bfmBB, bfmB2, bkd

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.9|Utilization of branched-chain amino acids]

[SW|Categories] containing this gene/protein

• [SW|utilization of amino acids]

This gene is a member of the following [SW|regulons]

• [SW|BkdR regulon], [SW|CodY regulon], [SW|SigL regulon]

Gene

Coordinates on the chromosome (coding sequence): 2,496,796 -> 2,498,070

The protein

Catalyzed reaction/ biological activity

• 2-methylpropanoyl-CoA enzyme N(6)-(dihydrolipoyl)lysine = CoA enzyme N(6)-(S-(2-methylpropanoyl)dihydrolipoyl)lysine (according to Swiss-Prot)

Protein family

• 2-oxoacid dehydrogenase family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|search|AcoC], [protein|search|OdhB], [protein|search|PdhC]

[SW|Cofactors]

• lipoic acid

[SW|Localization]

• Nucleoid (Heterogeneous) [Pubmed|16479537]

Expression and Regulation

Operon

• ''[protein|search|ptb]-[protein|search|bcd]-[protein|search|buk]-[protein|search|lpdV]-[SW|bkdAA]-[SW|bkdAB]-[protein|search|bkdB]'' [Pubmed|12823818]

[SW|Sigma factor]

• [protein|search|SigL] [Pubmed|10094682]

Regulation

• induced in the presence of isoleucine or valine ([protein|search|BkdR]) [Pubmed|10094682]

Regulatory mechanism

• [protein|search|CodY]: transcription repression [Pubmed|10094682]
• [protein|search|BkdR]: transcription activation [Pubmed|10094682]

References

• 12427936,15241682,10094682,10094682,16479537,12823818