etfB

electron transfer flavoprotein (beta subunit)

locus: BSU_28530

pI: 4.19

mw: 28.37 kDa

protein length: 257 aa

gene length: 774 bp

function: fatty acid degradation

product: electron transfer flavoprotein (beta subunit)

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.3|Electron transport/ other]
• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.3|Utilization of fatty acids]

Gene

Coordinates: 2,916,378 2,917,151

The protein

Protein family

• ETF beta-subunit/fixA family (single member, according to UniProt)

[SW|Cofactors]

• FAD (according to UniProt)

Structure

• [PDB|1EFP] (from Paracoccus denitrificans, 37% identity) [pubmed|10026281]

Expression and Regulation

Operons

• genes: [gene|7AC5217CA35F8E134ABB35D74F1BA5E684D2E058|lcfA]-[gene|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]-[gene|3111ECB66CD3CFF0494AA3DF4D646102CF3B0FA8|fadB]-[gene|251600E5FD52DC0352A123F1224C037577F5A09C|etfB]-[gene|ACA8FF923D72CC99F447E47F6A1381FEAD9DAB09|etfA]
  description: [Pubmed|17189250]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21398533], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]: repression, [Pubmed|17189250], in [regulon|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|21398533], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • induced by long chain acyl-CoA (C14 ... C20) ([protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]) [Pubmed|17189250]
  • expression of the operon is strongly induced during [SW|biofilm formation] [pubmed|31113899]

• genes: [gene|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]-[gene|3111ECB66CD3CFF0494AA3DF4D646102CF3B0FA8|fadB]-[gene|251600E5FD52DC0352A123F1224C037577F5A09C|etfB]-[gene|ACA8FF923D72CC99F447E47F6A1381FEAD9DAB09|etfA]
  description: [Pubmed|17189250]

  regulation

  • induced by long-chain fatty acids ([protein|search|FadR]) [Pubmed|17189250]

Biological materials

Mutant

• 1A855 ( ''etfB''::''cat''), [Pubmed|17085570], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A855&Search=1A855 BGSC]
• BKE28530 (''[gene|251600E5FD52DC0352A123F1224C037577F5A09C|etfB]''::''erm'', available in the BGSC, in [SW|Fabian Commichau]'s, and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
• BKE28530 ([gene|251600E5FD52DC0352A123F1224C037577F5A09C|etfB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAGATTCATGATCATATCCC, downstream forward: _UP4_TAAAACTTGGACATTCAAAG
• BKK28530 ([gene|251600E5FD52DC0352A123F1224C037577F5A09C|etfB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAGATTCATGATCATATCCC, downstream forward: _UP4_TAAAACTTGGACATTCAAAG

References

• 17189250,17919287,17085570,10026281