sqhC

squalene-hopene cyclase, biosynthesis of sporulenes, protection of the spore against oxidative stress

Locus: BSU_19320

Isoelectric point: 7.63

Molecular weight: 71.05 kDa

Protein length: 632 aa

Gene length: 1899 bp

Function: biosynthesis of sporulenes

Product: squalene-hopene cyclase

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
• Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress

Gene

Coordinates: 2,102,168 2,104,066

The protein

Catalyzed reaction/ biological activity

• sporulenol --> H₂O + tetraprenyl-β-curcumene (according to UniProt)
• production of sporulenes, polycyclic terpenoid lipids PubMed
• protection of the spore against oxidative stress PubMed

Protein family

• terpene cyclase/mutase family (single member, according to UniProt)

Structure

• 2SQC (from Alicyclobacillus acidocaldarius, 32% identity) PubMed

Localization

• surrounds the forespores PubMed

Expression and Regulation

Operons

• Genes: sqhC-sodF
  Description: PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

  Regulation

  • expressed during sporulation in the mother cell PubMed

Biological materials

Mutant

• BKE19320 (sqhC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCACGCTCAACTCCTTCA, downstream forward: _UP4_GATTCTATTGAAAAGGAGAC
• BKK19320 (sqhC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCACGCTCAACTCCTTCA, downstream forward: _UP4_GATTCTATTGAAAAGGAGAC

References

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