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sinI
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sinI

antagonist of [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR], drives [protein|920F91E748EE079FF864011D9052B073567C41E4|SlrR] from the [protein|920F91E748EE079FF864011D9052B073567C41E4|SlrR](LOW) to the [protein|920F91E748EE079FF864011D9052B073567C41E4|SlrR](HIGH) state
locus
BSU_24600
pI
6.33
mw
6.47 kDa
protein length
57 aa Sequence Blast
gene length
174 bp Sequence Blast
function
control of [SW|biofilm formation]
product
antagonist of [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProt

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.6|Transition state regulators]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.4|Regulation]

    • Gene

    Phenotypes of a mutant

  • altered cell death pattern in colonies [Pubmed|23012477]

    • The protein

    Paralogous protein(s)

  • [protein|C17C296F3EB2E106C380E3B5D784F3FA63F4C9B7|SlrA]

    • [SW|Domains]

  • [SW|Sin domain] (aa 2-40) (according to UniProt)

    • Structure

  • [PDB|5TMX] [pubmed|31493408]
  • [PDB|1B0N] (complex [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]-[protein|24D6A7DDCB25B99FBB670E9203F826596916B35C|SinI]) [Pubmed|9799632]

    • Expression and Regulation

    Operons

    genes
    [gene|24D6A7DDCB25B99FBB670E9203F826596916B35C|sinI]-[gene|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]
    description
    [Pubmed|3125149]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|3125149], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, in a portion of cells [Pubmed|11751836], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, [Pubmed|1664536], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [PubMed|7635837,11751836], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]: repression, [PubMed|1906467,11751836], in [regulon|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC regulon]

    • regulation

  • repressed during exponential growth ([protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1906467,11751836 PubMed]

    • additional information

  • the mRNA is stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
    • view in new tab

    Biological materials

    Mutant

  • GP959 (spc), available in [SW|Jörg Stülke]'s lab
  • DS91 (spc) NCIB3610 derivate, available in [SW|Jörg Stülke]'s lab
  • 1S98 ( ''sinI''::''kan''), [Pubmed|11466285], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S98&Search=1S98 BGSC]
  • GP1663 (''[gene|22BEB39F735DAAF71F1B382CD453A9C2D4CC9FA4|yqhG]-[gene|24D6A7DDCB25B99FBB670E9203F826596916B35C|sinI]-[gene|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]-[gene|BF97457E986656E4A9FE7A858F5BDF1759850D5C|tasA]''), available in [SW|Jörg Stülke]'s lab
  • BKE24600 ([gene|24D6A7DDCB25B99FBB670E9203F826596916B35C|sinI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCAGTTTCTCCTCCTAA, downstream forward: _UP4_TGAATGTGCTATAATATCAC
  • BKK24600 ([gene|24D6A7DDCB25B99FBB670E9203F826596916B35C|sinI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCAGTTTCTCCTCCTAA, downstream forward: _UP4_TGAATGTGCTATAATATCAC

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 20541494

    • Modelling of the [protein|24D6A7DDCB25B99FBB670E9203F826596916B35C|SinI]]/[[SinR] switch

  • 21095906

    • Original publications

  • 23430750,23012477,21326214,21815947,3125149,15661000,18047568,11751836,1906467,11751836,7635837,11751836,10547280,15104138,9799632,20923420,8422983,24256735,31493408,31604312