SubtiBank SubtiBank
metE
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metE

methionine synthase
Locus
BSU_13180
Isoelectric point
4.84
Molecular weight
86.64 kDa
Protein length
762 aa Sequence Blast
Gene length
2289 bp Sequence Blast
Function
biosynthesis of methionine
Product
methionine synthase
Essential
no
E.C.
2.1.1.14
Synonyms
metC

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,383,320 1,385,608

The protein

Catalyzed reaction/ biological activity

  • 5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine --> L-methionine + tetrahydropteroyltri-L-glutamate (according to UniProt)
  • Protein family

  • vitamin-B12 independent methionine synthase family (single member, according to UniProt)
  • Modification

  • phosphorylated on ser/ thr/ tyr PubMed, S-cysteinylation after diamide stress (C719) PubMed
  • Cys719 and Cys730 are S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed PubMed
  • MetE is generally most strongly S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed PubMed
  • Structure

  • 1T7L (from Thermotoga maritima, 44% identity, 61% similarity) PubMed
  • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • belongs to the 100 most abundant proteins PubMed
  • Expression and Regulation

    Operons

    Genes

    Regulatory mechanism

  • S-box: termination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box
  • Regulation

  • repressed by casamino acids PubMed
  • the S-box RNA is degraded by RNase Y PubMed
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    Biological materials

    Mutant

  • 1A607 ( metE::erm), PubMed, available at BGSC
  • BKE13180 (metE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGTTTCTCCTCCTTTAT, downstream forward: _UP4_TAATTTGAAAAAACCATCTG
  • BKK13180 (metE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGTTTCTCCTCCTTTAT, downstream forward: _UP4_TAATTTGAAAAAACCATCTG
  • References

    Reviews

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    Original Publications

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