metE

methionine synthase

Locus: BSU_13180

Isoelectric point: 4.84

Molecular weight: 86.64 kDa

Protein length: 762 aa

Gene length: 2289 bp

Function: biosynthesis of methionine

Product: methionine synthase

Essential: no

E.C.: 2.1.1.14

Synonyms: metC

Genomic Context

Loading

Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of methionine/ S-adenosylmethionine
• Groups of genes → Phosphoproteins → Phosphorylation on either a Ser, Thr or Tyr residue

Gene

Coordinates: 1,383,320 1,385,608

The protein

Catalyzed reaction/ biological activity

• 5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine --> L-methionine + tetrahydropteroyltri-L-glutamate (according to UniProt)

Protein family

• vitamin-B12 independent methionine synthase family (single member, according to UniProt)

Modification

• phosphorylated on ser/ thr/ tyr PubMed, S-cysteinylation after diamide stress (C719) PubMed
• Cys719 and Cys730 are S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed PubMed
• MetE is generally most strongly S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed PubMed

Structure

• 1T7L (from Thermotoga maritima, 44% identity, 61% similarity) PubMed

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed
• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: metE

  Regulatory mechanism

  • S-box: termination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box

  Regulation

  • repressed by casamino acids PubMed
  • the S-box RNA is degraded by RNase Y PubMed

Biological materials

Mutant

• 1A607 ( metE::erm), PubMed, available at BGSC
• BKE13180 (metE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGTTTCTCCTCCTTTAT, downstream forward: _UP4_TAATTTGAAAAAACCATCTG
• BKK13180 (metE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGTTTCTCCTCCTTTAT, downstream forward: _UP4_TAATTTGAAAAAACCATCTG

References

Reviews

Loading

Original Publications

Loading