gcaD

bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

Locus: BSU_00500

Isoelectric point: 5.65

Molecular weight: 49.29 kDa

Protein length: 456 aa

Gene length: 1371 bp

Function: cell wall metabolism

Product: bifunctional N-acetylglucosamine-1-phosphate

Essential: yes

E.C.: 2.7.7.23

Synonyms: tms,tms26,glmU

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall synthesis → Biosynthesis of peptidoglycan
• Metabolism → Additional metabolic pathways → Biosynthesis of cell wall components → Biosynthesis of peptidoglycan
• Groups of genes → Essential genes

Gene

Coordinates: 56,352 57,722

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• acetyl-CoA + α-D-glucosamine 1-phosphate --> CoA + H⁺ + N-acetyl-α-D-glucosamine 1-phosphate (according to UniProt)
• H⁺ + N-acetyl-α-D-glucosamine 1-phosphate + UTP --> diphosphate + UDP-N-acetyl-α-D-glucosamine (according to UniProt)

Protein family

• N-terminal domain: N-acetylglucosamine-1-phosphate uridyltransferase family (single member, according to UniProt)
• C-terminal domain: Transferase hexapeptide repeat family (according to UniProt)

Structure

• 1HM9 (from Streptococcus pneumoniae, 49% identity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: gcaD-prs-ctc
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • stringent response: negative regulation, PubMed, in stringent response

Biological materials

Expression vectors

• pGP2596: (IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab

References

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