SubtiBank SubtiBank
queA
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queA

S-adenosylmethionine tRNA ribosyltransferase
Locus
BSU_27720
Isoelectric point
5.05
Molecular weight
38.35 kDa
Protein length
342 aa Sequence Blast
Gene length
1029 bp Sequence Blast
Function
tRNA modification
Product
S-adenosylmethionine tRNA ribosyltransferase
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation

    • Gene

    Coordinates
    2,833,899 2,834,927

    The protein

    Catalyzed reaction/ biological activity

  • 7-aminomethyl-7-carbaguanosine34 in tRNA + S-adenosyl-L-methionine --> adenine + epoxyqueuosine34 in tRNA + H+ + L-methionine (according to UniProt)

    • Protein family

  • queA family (single member, according to UniProt)

    • Structure

  • 1YY3 PubMed

    • Expression and Regulation

    Operons

    Genes
    ruvA-ruvB-yrzS-queA-tgt-yrbF
    Description
    PubMed

    Regulation

  • the mRNA is processed between tgt and yrbF by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed
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    Biological materials

    Mutant

  • BKE27720 (queA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATGATTTCACCTTTAT, downstream forward: _UP4_TAATTTGAGAACAGGAGGCC
  • BKK27720 (queA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATGATTTCACCTTTAT, downstream forward: _UP4_TAATTTGAGAACAGGAGGCC

    • References

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