yvaE [2019-06-12 10:09:48]

similar to multidrug-efflux transporter

locus: BSU33570

pI: 8.13

mw: 12.82 kDa

protein length: 119 aa

gene length: 360 bp

function: unknown

product: subunit of unidentified multidrug transporter

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.14|Multidrug exporters]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.14|Resistance against toxins/ antibiotics/ based on similarity]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,448,693 3,449,052

The protein

Protein family

• paired small multidrug resistance protein family ([SW|PSMR family]) [Pubmed|17942072]

Structure

• [PDB|3B5D] (from E. coli, 44% identity) [pubmed|18024586]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|97CE9735931D02E6E780C955C9BE3A67E5C4C59A|yvaF]-[gene|230D790937DAFB82A04B8597699115E1C1EAE98E|yvaE]-[gene|B7FBC30B744D8B1F26AFC5DCB297FAFAB5C60FB7|yvaD]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-A502 (yvaE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/502 NBRP B. subtilis, Japan]
• BKE33570 ([gene|230D790937DAFB82A04B8597699115E1C1EAE98E|yvaE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGCCAAGCATAGAAACACCC, downstream forward: _UP4_GTACATAAAAGCGGGCAGTA
• BKK33570 ([gene|230D790937DAFB82A04B8597699115E1C1EAE98E|yvaE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGCCAAGCATAGAAACACCC, downstream forward: _UP4_GTACATAAAAGCGGGCAGTA

References

Reviews

• 17942072

Original publications

• 21926231,18024586