accA

acetyl-CoA carboxylase (alpha subunit)

locus: BSU_29200

pI: 6.09

mw: 36.18 kDa

protein length: 325 aa

gene length: 978 bp

function: production of malonyl-CoA, the substrate for fatty acid biosynthesis

product: acetyl-CoA carboxylase (alpha subunit))

essential: yes

ec: 6.4.1.2

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 2,987,731 2,988,708

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• acetyl-CoA + N⁶-carboxybiotinyl-L-lysyl-[protein] --> malonyl-CoA + N⁶-biotinyl-L-lysyl-[protein] (according to UniProt)

Protein family

• accA family (single member, according to UniProt)

[SW|Domains]

• CoA carboxyltransferase C-terminal domain (aa 38-292) (according to UniProt)

Structure

• [PDB|2F9I] (from ''Staphylococcus aureus''; equivalent to [protein|E4997526CC534E38413030E90F7A135ECF65DF40|AccD]-[protein|22B3039DF4D106F121DBC765F49878C9B324FDB2|AccA], 64% identity) [Pubmed|16460018]

[SW|Localization]

• cytoplasm (according to Swiss-Prot), Membrane-proximal (Spotty) [Pubmed|16479537]

Expression and Regulation

Operons

• genes: [gene|E4997526CC534E38413030E90F7A135ECF65DF40|accD]-[gene|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

  regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

Biological materials

Expression vectors

• pGP1021: purification from ''E. coli'' with an N-terminal His-tag, with TEV cleavage site, (in [SW|pETM-11]), available in [SW|Jörg Stülke]'s lab
• pGP1024: purification from ''E. coli'' with an N-terminal Strep-tag, with TEV cleavage site, (in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
• pGP1723: purification from ''B. subtilis'' with an C-terminal Strep-tag, for [SW|SPINE], (in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab

GFP fusion

• GP1490 (spc, based on [SW|pBP43]), available in [SW|Jörg Stülke]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

FLAG-tag construct

• GP1487 (''spc'', based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

References

Reviews

• 15952903,17919287,12121720,15968460

Original Publications

• 15066985,16460018,14651647,16479537,12663926,22383849,28579978,16460018,27471863