ccpN [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]

transcriptional repressor (CcpN family) of gluconeogenetic genes and of sr1. repression in the presence of glucose

Locus: BSU25250

Isoelectric point: 7.00

Molecular weight: 23.00 kDa

Function: repressor of genes involved in
gluconeogenesis (gapB, pckA) and of sr1

Product: transcriptional regulator (CcpN family)

Essential: no

Synonyms: yqzB

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Carbon core metabolism → Gluconeogenesis
• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other
• Information processing → Regulation of gene expression → Regulators of core metabolism

Categories containing this gene/protein

• carbon core metabolism, transcription factors and their control, regulators of core metabolism

Gene

Coordinates on the chromosome (coding sequence): 2,604,959 -> 2,605,597

Phenotypes of a mutant

• Impaired growth on glucose due to re-routing of carbon from glycolysis to the pentose phosphate pathway PubMed

The protein

Catalyzed reaction/ biological activity

• transcription repression of the gapB, pckA, and sr1 genes in the presence of glucose PubMed

Protein family

• CcpN family

Domains

• 2 CBS domains (83-139, 148-211)

Effectors of protein activity

• YqfL (negative effector of CcpN activity) PubMed
• ATP enhances CcpN-dependent repression, ADP counteracts the repressing activity of CcpN PubMed

Interactions

• active as dimer (according to PubMed)
• CcpN-RpoA PubMed

Expression and Regulation

Operon

• ccpN-yqfL PubMed

Regulation

• constitutively expressed PubMed

Additional information

• the intracellular concentration of CcpN is about 4 myM (according to PubMed).

Biological materials

Mutant

• DB104 ccpN::cat, available in Sabine Brantl's lab
• GP1128 ccpN::cat, available in Jörg Stülke's lab

Expression vector

• pGP2923 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab

Antibody

• available in Sabine Brantl's lab

Labs working on this gene/protein

• Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
• Sabine Brantl, Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany homepage
• Uwe Sauer, ETH Zürich, Switzerland homepage

References

Reviews

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Original Publications

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