ytnA

alanine permease

Locus: BSU_30530

Isoelectric point: 9.91

Molecular weight: 50.16 kDa

Protein length: 463 aa

Gene length: 1392 bp

Function: uptake of D- and L-alanine

Product: alanine permease

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Amino acid transporters → APC superfamily
• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/acquisition of L- and D-alanine
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,124,250 3,125,641

Phenotypes of a mutant

• a ytnA alr double mutant is not viable due to the inability to acquire D-alanine reference

The protein

Catalyzed reaction/ biological activity

• uptake of D- and L-alanine reference

Protein family

• amino acid-polyamine-organocation (APC) superfamily (according to UniProt)

Paralogous protein(s)

• HutM, YvbW, YbxG, RocC, RocE, YbgF, YdgF, AapA, GabP

Structure

• 6F34 (from Geobacillus kaustophilus, 21% identity) PubMed

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: asnB-ytnA
  Description: PubMed

  Regulation

  • constitutive

Biological materials

Mutant

• MGNA-A126 (ytnA::erm), available at the NBRP B. subtilis, Japan
• GP1885 (ΔytnA::spc) available in Jörg Stülke's lab PubMed
• BKE30530 (ytnA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTTCTTCTCCCCTAGA, downstream forward: _UP4_TGACAAAAAGAGCTCCCAGT
• BKK30530 (ytnA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTTCTTCTCCCCTAGA, downstream forward: _UP4_TGACAAAAAGAGCTCCCAGT

Expression vector

• pGP2280: expression of ytnA by pBQ200 in B. subtilis, available in Jörg Stülke's lab

LacZ fusion

• pGP2276 (in pAC5) (GP2963), available in Jörg Stülke's lab

References

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