SubtiBank SubtiBank
ytnA
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ytnA

alanine permease
Locus
BSU_30530
Isoelectric point
9.91
Molecular weight
50.16 kDa
Protein length
463 aa Sequence Blast
Gene length
1392 bp Sequence Blast
Function
uptake of D- and L-alanine
Product
alanine permease
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,124,250 3,125,641

Phenotypes of a mutant

  • a ytnA alr double mutant is not viable due to the inability to acquire D-alanine reference
  • The protein

    Catalyzed reaction/ biological activity

  • uptake of D- and L-alanine reference
  • Protein family

    Paralogous protein(s)

    Structure

  • 6F34 (from Geobacillus kaustophilus, 21% identity) PubMed
  • Localization

  • cell membrane (according to UniProt)
  • Expression and Regulation

    Operons

    Genes
    Description

    Regulation

  • constitutive
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    Biological materials

    Mutant

  • MGNA-A126 (ytnA::erm), available at the NBRP B. subtilis, Japan
  • GP1885 (ΔytnA::spc) available in Jörg Stülke's lab PubMed
  • BKE30530 (ytnA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTTCTTCTCCCCTAGA, downstream forward: _UP4_TGACAAAAAGAGCTCCCAGT
  • BKK30530 (ytnA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTTCTTCTCCCCTAGA, downstream forward: _UP4_TGACAAAAAGAGCTCCCAGT
  • Expression vector

  • pGP2280: expression of ytnA by pBQ200 in B. subtilis, available in Jörg Stülke's lab
  • LacZ fusion

  • pGP2276 (in pAC5) (GP2963), available in Jörg Stülke's lab
  • References

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